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Expression of BORIS in melanoma: lack of association with MAGE-A1 activation.

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Kholmanskikh, Olga [UCL] Loriot, Axelle [UCL] Brasseur, Francis De Plaen, Etienne [UCL] De Smet, Charles [UCL]

Several genes with specific expression in germ cells show aberrant activation in different types of tumors. These genes, termed cancer-germline (CG) genes, encode tumor-specific antigens, which represent potential targets for therapeutic vaccination against cancer. The germline-specific gene BORIS (Brother Of the Regulator of Imprinted Sites), which encodes an 11-zinc-fingers transcriptional regulator, was recently qualified as a new CG gene, as it was found to be activated in a variety of tumor samples. Moreover, it was suggested that BORIS might be responsible for the activation of most other CG genes, including gene MAGE-A1, in tumors. In the present study, we evaluated the frequency of BORIS activation in melanoma by quantitative RT-PCR. BORIS activation was detected in 27% (n = 63) melanoma tissue samples. Surprisingly, many melanoma samples expressed MAGE-A1 and other CG genes in the absence of BORIS activation, suggesting that BORIS is not an obligate factor for activation of these genes in melanoma. Consistently, forced expression of BORIS in melanoma cell lines did not induce expression of MAGE-A1. Our results indicate that BORIS may serve as a useful target for immunotherapy of melanoma. However, it appears that BORIS is neither necessary nor sufficient for the activation of other CG genes.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2008
Journal information "International journal of cancer. Journal international du cancer" - Vol. 122, no. 4, p. 777-84 (2008)
Peer reviewed yes
issn 0020-7136
e-issn 1097-0215
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Antigens, Neoplasm - genetics - metabolism ; Azacitidine ; Blotting, Western ; CpG Islands ; DNA Methylation ; DNA-Binding Proteins - genetics - metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Melanoma - genetics - metabolism - pathology ; Neoplasm Proteins - genetics - metabolism ; RNA, Messenger - genetics - metabolism ; Retroviridae - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Skin Neoplasms - genetics - metabolism - pathology ; Transfection ; Tumor Cells, Cultured
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Bibliographic reference Kholmanskikh, Olga ; Loriot, Axelle ; Brasseur, Francis ; De Plaen, Etienne ; De Smet, Charles. Expression of BORIS in melanoma: lack of association with MAGE-A1 activation.. In: International journal of cancer. Journal international du cancer, Vol. 122, no. 4, p. 777-84 (2008)
Permanent URL http://hdl.handle.net/2078.1/11164