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Localization of GAD-like immunoreactivity in the pancreas and stomach of the rat and mouse

Bibliographic reference Gilon, Patrick ; Tappaz, M. ; Remacle, Claude. Localization of GAD-like immunoreactivity in the pancreas and stomach of the rat and mouse. In: Histochemistry, Vol. 96, no. 4, p. 355-365 (1991)
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  1. Baekkeskov S, Aanstoot HJ, Christgau S, Reetz A, Solimena M, Cascalho M, Folli F, Richter-Olesen H, De Camilli P (1990) Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature 347:151?156
  2. Berod A, Hartman BK, Pujol JF (1981) Importance of fixation in immunohistochemistry: use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase. J Histochem Cytochem 29:844?850
  3. Davanger S, Ottersen OP, Storm-Mathisen J (1987) Immunocytochemical localization of GABA in cat myenteric plexus. Neurosci Lett 73:27?32
  4. Davanger S, Ottersen OP, Storm-Mathisen J (1989) GABA-immunoreactive cells in the rat gastrointestinal epithelium. Anat Embryol 179:221?226
  5. Erdö SL, Wolff JR (1988) Releasable, non-neuronal GABA pool in rat stomach. Eur J Pharmacol 156:165?168
  6. Erdö SL, Ezer E, Matuz J, Wolff JR, Amenta F (1989) GABAA receptors in the rat stomach may mediate mucoprotective effects. Eur J Pharmacol 165:79?86
  7. Erdö SL, De Vincentis G, Amenta F (1990) Autoradiographic localization of [3H]muscimol binding sites in rat stomach: evidence for mucosal GABAA receptors. Eur J Pharmacol 175:351?354
  8. Gamrani H, Harandi M, Belin MF, Dubois MP, Calas A (1984) Direct electron microscopic evidence for the coexistence of GABA uptake and endogenous serotonin in the same rat central neurons by coupled radioautographic and immunocytochemical procedures. Neurosci Lett 48:25?30
  9. Garry DJ, Sorenson RL, Coulter HD (1987a) Ultrastructural localization of gamma amino butyric acid immunoreactivity in B cells of the rat pancreas. Diabetologia 30:115?119
  10. Garry DJ, Coulter HD, MacIntee TJ, Wu JY, Sorenson RL (1987b) Immunoreactive GABA transaminase within the pancreatic islet is localized in mitochondria of the B-cell. J Histochem Cytochem 35:831?836
  11. Garry DJ, Garry MG, Sorenson RL (1988) Ultrastructural immunocytochemical localization of l-glutamate decarboxylase and GABA in rat pancreatic zymogen granules. Cell Tissue Res 252:191?197
  12. Gilon P (1990) Le GABA et les cellules endocrines du système gastro-entéro-pancréatique. Thesis, University of Louvain, Louvain-la-Neuve
  13. Gilon P, Remacle C, Janssens de Varebeke P, Pauwels G, Hoet JJ (1987a) GABA content and localization of high-affinity GABA uptake during the development of the rat pancreas. Cell Mol Biol 33:573?585
  14. Gilon P, Reusens-Billen B, Remacle C, Janssens de Varebeke P, Pauwels G, Hoet JJ (1987b) Localization of high-affinity GABA uptake and GABA content in the rat duodenum during development. Cell Tissue Res 249:593?600
  15. Gilon P, Campistron G, Geffard M, Remacle C (1988) Immunocytochemical localization of GABA in endocrine cells of the rat entero-pancreatic system. Biol Cell 62:265?273
  16. Gilon P, Remacle C (1989) High-affinity GABA uptake in a subpopulation of somatostatin cells in rat pancreas. J Histochem Cytochem 37:1133?1139
  17. Gilon P, Mallefet J, De Vriendt C, Pauwels S, Geffard M, Campistron G, Remacle C (1990) Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach. Histochemistry 93:645?654
  18. Gottlieb DI, Chang YC, Schwob JE (1986) Monoclonal antibodies to glutamic acid decarboxylase. Proc Natl Acad Sci USA 83:8808?8812
  19. Guo YS, Thompson JC, Singh P (1989) Effect of ?-aminobutyric acid on bombesin-evoked release of somatostatin and gastrin from isolated rat stomach. Regul Pep 24:179?186
  20. Hakanson R, Alumets J, Stewart JA, Sundler F (1978) Does glutamate decarboxylase occur in endocrine cells of gut and pancreas? Scand J Gastroenterol 13 [Suppl 49]:74
  21. Harty RF, Franklin PA (1983) GABA affects the release of gastrin and somatostatin from rat antral mucosa. Nature 303:623?624
  22. Harty RF, Franklin PA (1986) Cholinergic mediation of ?-aminobutyric acid-induced gastrin and somatostatin release from rat antrum. Gastroenterology 91:1221?1226
  23. Harty RF, Murthy SNS (1986) GABAergic mechanisms in the endocrine stomach and duodenum: their possible functional significance. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery. Raven Press, New York, pp 339?352
  24. Harty RF, Carr T, Boharski MG, Lassiter DC, Egan PT (1986) Cholinergic and ?-aminobutyric acid neurotransmitter release from antral mucosal and submucosal neurons. Can J Physiol Pharmacol (Suppl) 64:39
  25. Hills JM, Jessen KR, Mirsky R (1987) An immunohistochemical study of the distribution of enteric GABA-containing neurons in the rat and guinea-pig intestine. Neuroscience 22:301?312
  26. Hougaard Dm, Nielsen JH, Larsson LI (1986) Localization and biosynthesis of polyamines in insulin-producing cells. Biochem J 238:43?47
  27. Jesen KR, Mirsky R, Hills JM (1987) GABA as an autonomic neurotransmitter: studies on intrinsic GABAergic neurons in the myenteric plexus of the gut. TINS 10:255?262
  28. Jessen KR, Hills JM, Limbrick AR (1988) GABA immunoreactivity and 3H-GABA uptake in mucosal epithelial cells of the rat stomach. Gut 29:1549?1556
  29. Klosen P, van den Bosch de Aguilar P (1990) Polyethylene-glycol sections for immunocytochemistry of neural antigens: a reappraisal. Eur J Neurosci [Suppl] 3:249
  30. Koop H, Arnold R (1986) Control of rat gastric somatostatin release by ?-aminobutyric acid (GABA). Horm Metab Res 18:94?97
  31. Krantis A, Webb T (1989) Autoradiographic localization of [3H] ?-aminobutyric acid in neuronal elements of the rat gastric antrum and intestine. J Auton Nerv Sys 29:41?48
  32. Lechago J (1987) The endocrine cells of the digestive tract. General concepts and historic perspective. Am J Surg Pathol 11 [Suppl 1]:63?70
  33. Legay F, Pelhate S, Tappaz ML (1986) Phylogenesis of brain glutamic acid decarboxylase from vertebrates: immunochemical studies. J Neurochem 46:1478?1486
  34. Legay F, Henry S, Tappaz ML (1987a) Monoclonal antibodies against rat brain glutamic acid decarboxylase (GAD). Neurochem Int 10:287?294
  35. Legay F, Henry S, Tappaz ML (1987b) Evidence for two distinct forms of native glutamic acid decarboxylase in rat brain soluble extract: an immunoblotting study. J Neurochem 48:1022?1026
  36. Li CY, Ziesmer SC, Lazcano-Villareal O (1987) Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method. J Histochem Cytochem 35:1457?1460
  37. Neale EA, Oertel WH, Bowers LM, Weise VK (1983) Glutamate decarboxylase immunoreactivity and ?-(3H)aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex. J Neurosci 3:376?382
  38. Oertel WH, Schmechel DE, Tappaz ML, Kopin IJ (1981a) Production of a specific antiserum to rat brain glutamic acid decarboxylase by injection of an antigen-antibody complex. Neuroscience 6:2689?2700
  39. Oertel WH, Schmechel DE, Mugnaini E, Tappaz ML, Kopin IJ (1981b) Immunocytochemical localization of glutamate decarboxylase in rat cerebellum with a new antiserum. Neuroscience 6:2715?2735
  40. Okada Y (1979) Properties of glutamate decarboxylase (GAD) in the human insulinoma, a non-neural tissue. Proc Jpn Acad 55:514?517
  41. Okada Y (1986) Localization and function of GABA in the pancreatic islets. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery. Raven Press, New York, pp 223?240
  42. Okada Y, Taniguchi H, Baba S (1982) High concentration of GABA in the pancreatic islets with special emphasis on B cells. In: Okada Y, Roberts E (eds) Problems in GABA research from brain to bacteria. Excerpta Medica, Amsterdam, pp 379?386
  43. Rahier J, Pauwels S, Dockray GJ (1987) Biosynthesis of gastrin. Localization of the precursor and peptide products using electron microscopic-immunogold methods. Gastroenterology 92:1146?1152
  44. Reisert I, Wöhrle M, Pilgrim C (1985) Quantitative assessment of GABA-uptake sites in the neural lobe by electron-microscopic autoradiography. Cell Tissue Res 241:581?584
  45. Rorsman P, Berggren PO, Bokvist K, Ericson H, Möhler H, Östenson CG, Smith PA (1989) Glucose-inhibition of glucagon secretion involves activation of GABAA-receptor chloride channels. Nature 341:233?236
  46. Sakanaka M, Shibasaki T, Lederis K (1987) Improved fixation and cobalt-glucose oxidase-diaminobenzidine intensification for immunohistochemical demonstration of corticotropin-releasing factor in rat brain. J Histochem Cytochem 35:207?212
  47. Sakaue M, Saito N, Tanaka C (1987) Immunohistochemical localization of gamma-aminobutyric acid (GABA) in the rat pancreas. Histochemistry 86:365?369
  48. Solcia E, Usellini L, Buffa R, Rindi G, Villani L, Zampatti C, Silini E (1987) Endocrine cells producing regulatory peptides. Experientia 43:839?850
  49. Staines WA, Meister B, Melander T, Nagy JI, Hökfelt T (1988) Three-color immunofluorescence histochemistry allowing triple labeling within a single section. J Histochem Cytochem 36:145?151
  50. Sternberger L A, Joseph S A, The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal., 10.1177/27.11.92498
  51. Taniguchi H, Murakami K, Yoshioka M, Ejiri K, Ishihara K, Baba S, Okada Y (1982a) GABA and insulin in pancreatic islets. In: Okada Y, Roberts E (eds) Problems in GABA research from brain to bacteria. Excerpta Medica, Amsterdam, pp 387?405
  52. Taniguchi H, Yoshioka M, Ejiri K, Ishihara K, Tamagawa M, Hirose Y, Ishihara K, Utsumi M, Baba S, Okada Y (1982b) Suppression of somatostatin release and increase of somatostatin content in pancreatic islets by GABA. In: Okada Y, Roberts E (eds) Problems in GABA research from brain to bacteria. Excerpta Medica, Amsterdam, pp 406?412
  53. Tappaz ML, Wassef M, Oertel WH, Paut L, Pujol JF (1983) Light- and electronmicroscopic immunocytochemistry of glutamic acid decarboxylase (GAD) in the basal hypothalamus: morphological evidence for neuroendocrine ?-aminobutyrate (GABA). Neuroscience 9:271?287
  54. Thirbly RC, Stevens MH, Blair AJ, Petty F, Crawford IL, Taylor IL, Walsh JH, Feldman M (1988) Effect of GABA on basal and vagally mediated gastric acid secretion and hormone release in dogs. Am J Physiol 254:G723-G731
  55. Vincent SR, Hökfelt T, Wu JY, Elde RP, Morgan LM, Kimmel JR (1983) Immunohistochemical studies of the GABA system in the pancreas. Neuroendocrinology 36:197?204