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Increased p70s6k phosphorylation during intake of a protein-carbohydrate drink following resistance exercise in the fasted state

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Deldicque, Louise [UCL] De Bock, Katrien [KUL] Maris, Michael [KUL] Ramaekers, Monique [KUL] Nielens, Henri [UCL] Francaux, Marc [UCL] Hespel, Peter [KUL]

The present study aimed at comparing the responses of myogenic regulatory factors and signaling pathways involved in muscle protein synthesis after a resistance training session performed in either the fasted or fed state. According to a randomized crossover study design, six young male subjects participated in two experimental sessions separated by 3 weeks. In each session, they performed a standardized resistance training. After the sessions, they received during a 4-h recovery period 6 ml/kg b.w. h of a solution containing carbohydrates (50 g/l), protein hydrolysate (33 g/l), and leucine (16.6 g/l). On one occasion, the resistance exercise session was performed after the intake of a carbohydrate-rich breakfast (B), whereas in the other session they remained fasted (F). Needle biopsies from m. vastus lateralis were obtained before (Rest), and 1 h (+1h) and 4 h (+4h) after exercise. Myogenin, MRF4, and MyoD1 mRNA contents were determined by RT-PCR. Phosphorylation of PKB (protein kinase B), GSK3, p70(s6k) (p70 ribosomal S6 kinase), eIF2B, eEF2 (eukaryotic elongation factor 2), ERK1/2, and p38 was measured via western blotting. Compared with F, the pre-exercise phosphorylation states of PKB and p70(s6k) were higher in B, whereas those of eIF2B and eEF2 were lower. During recovery, the phosphorylation state of p70(s6k) was lower in B than in F (p = 0.02). There were no differences in basal mRNA contents between B and F. However, compared with F at +1h, MyoD1 and MRF4 mRNA contents were lower in B (p < 0.05). Our results indicate that prior fasting may stimulate the intramyocellular anabolic response to ingestion of a carbohydrate/protein/leucine mixture following a heavy resistance training session.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2010
Language Anglais
Journal information "European Journal of Applied Physiology" - Vol. 108, no. 4, p. 791-800 (2010)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 1439-6319
e-issn 1439-6327
Publication status Publié
Affiliations UCL - SSS/IONS - Institute of NeuroScience
KUL
UCL - (SLuc) Service de médecine physique et de réadaptation motrice
UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
MESH Subject Algorithms ; Blood Chemical Analysis ; Male ; Phosphorylation - drug effects ; Protein Kinases - metabolism ; Resistance Training ; Ribosomal Protein S6 Kinases, 70-kDa - metabolism ; Up-Regulation ; Young Adult ; Cross-Over Studies ; Dietary Carbohydrates - administration & dosage, pharmacology ; Dietary Proteins - administration & dosage, pharmacology ; Drinking - physiology ; Exercise Test ; Fasting - physiology ; Feeding Methods ; Humans
Links
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Bibliographic reference Deldicque, Louise ; De Bock, Katrien ; Maris, Michael ; Ramaekers, Monique ; Nielens, Henri ; et. al. Increased p70s6k phosphorylation during intake of a protein-carbohydrate drink following resistance exercise in the fasted state. In: European Journal of Applied Physiology, Vol. 108, no. 4, p. 791-800 (2010)
Permanent URL http://hdl.handle.net/2078.1/110107