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Tissue factor-dependent procoagulant activity of isolated human hepatocytes: relevance to liver cell transplantation.

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Document type : Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Abstract :

Liver cell transplantation (LCT) aims to correct inborn liver function defects by infusing metabolically active cells into the diseased liver. Further improvement in LCT might depend on the prevention of early loss of transplanted cells. As tissue factor (TF)-dependent activation of coagulation was found to contribute to a low rate of beta cell engraftment in islet transplantation, we investigated the potential procoagulant activity (PCA) of hepatocyte preparations. TF expression on hepatocyte preparations was assessed by flow cytometry, reverse-transcription polymerase chain reaction and immunofluorescence. PCA depending on TF was evaluated in human plasma and in whole blood systems. Coagulation parameters were followed by routine techniques in a LCT recipient Crigler-Najjar patient. We determined that hepatocytes express soluble and membrane-bound forms of TF. We showed that hepatocytes exert a TF-dependent PCA. In parallel, delayed increase in D-dimer levels was observed following the hepatocyte infusions in the Crigler-Najjar patient. Furthermore, in vitro experiments demonstrated that TF-dependent PCA of hepatocytes is inhibited by N-acetyl-L-cysteine. In conclusion, hepatocytes exert TF-dependent PCA, which may contribute to early loss of infused cells. Addition of N-acetyl-L-cysteine to the suspensions of hepatocytes might be beneficial in LCT by inhibiting activation of coagulation.
Access type : Accès restreint
Publication date : 2007
Journal information : "Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society" - Vol. 13, no. 4, p. 599-606 (2007)
Peer reviewed : yes
issn : 1527-6465
Publication status : Publié
Affiliations : UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie
UCL - (SLuc) Centre de thérapie tissulaire et cellulaire
MESH Subject : Blood Coagulation ; Cell Transplantation - methods ; Cryopreservation ; Hepatocytes - cytology - drug effects - physiology ; Humans ; Liver Transplantation - physiology ; RNA, Messenger - genetics ; Thromboplastin - genetics - physiology
Links :
Bibliographic reference Stéphenne, Xavier ; Vosters,Olivier ; Najimi, Mustapha ; Beuneu, Claire ; Dung, Khuu Ngoc ; et. al. Tissue factor-dependent procoagulant activity of isolated human hepatocytes: relevance to liver cell transplantation.. In: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 13, no. 4, p. 599-606 (2007)
Permanent URL http://hdl.handle.net/2078.1/10911

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