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Expression and secretion of recombinant outer-surface protein A from the Lyme disease agent, Borrelia burgdorferi, in Nicotiana tabacum suspension cells.

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Navarre, Catherine [UCL] Delannoy, Mélanie [UCL] Lefebvre, Benoit Nader, Joseph [UCL] Vanham, Delphine Boutry, Marc [UCL]

The ospA gene of Borrelia burgdorferi codes for an outer membrane lipoprotein, which is a major antigen of the Lyme disease agent. Recombinant OspA vaccines tested so far were expressed in Escherichia coli. In this study, we investigated the expression of a soluble OspA protein in Nicotiana tabacum suspension cells and evaluated the secretion of OspA driven by either its own bacterial signal peptide or a plant signal peptide fused to the amino-terminal cysteine of the mature form. In both cases, the signal peptide was cleaved off and OspA secreted. During secretion, OspA was N-glycosylated. Addition of a C-terminal KDEL sequence led to retention of OspA in the endoplasmic reticulum.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2006
Language Anglais
Journal information "Transgenic research" - Vol. 15, no. 3, p. 325-35 (2006)
Peer reviewed yes
issn 0962-8819
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Antigens, Surface - chemistry - genetics ; Bacterial Outer Membrane Proteins - chemistry - genetics ; Bacterial Vaccines - chemistry - genetics ; Borrelia burgdorferi - metabolism ; Endoplasmic Reticulum - metabolism ; Escherichia coli - metabolism ; Gene Expression Regulation, Plant ; Genetic Techniques ; Genetic Vectors ; Glycosylation ; Lipoproteins - chemistry - genetics ; Models, Genetic ; Plant Diseases - genetics - microbiology ; Plants, Genetically Modified ; Protein Processing, Post-Translational ; Protein Structure, Tertiary ; Tobacco - metabolism - microbiology ; Transgenes
Links
  1. G An, Plant Physiol, 79, 568 (1985)
  2. H Batoko, Plant Cell, 12, 2201 (2000)
  3. I Benz, Mol Microbiol, 45, 267 (2002)
  4. B Bouchon, Anal Biochem, 246, 52 (1997)
  5. M Bradford, Anal Biochem, 72, 248 (1976)
  6. AJ Crofts, Plant Cell, 11, 2233 (1999)
  7. JJ Dunn, Protein Expr Purif, 1, 159 (1990)
  8. LF Erdile, Infect Immun, 61, 81 (1993)
  9. E Fikrig, Infect Immun, 60, 773 (1992)
  10. W Golde, Borrelia burgdorferi Infect Immun, 65, 882 (1997)
  11. T Häupl, FEMS Immun Med Microbiol, 19, 15 (1997)
  12. S Hellwig, Nat Biotechnol, 22, 1415 (2004)
  13. BJB Johnson, Vaccine, 13, 1086 (1995)
  14. Kawaguchi R, Regulation of translational initiation in plants, 10.1016/s1369-5266(02)00290-x
  15. P Lerouge, Plant Mol Biol, 38, 31 (1998)
  16. BJ Luft, Borrelia burgdorferi J Infec Dis, 185, 46 (2002)
  17. M Lukaszewicz, Plant Sci, 154, 89 (2000)
  18. Mitsuhara I., Ugaki M., Hirochika H., Ohshima M., Murakami T., Gotoh Y., Katayose Y., Nakamura S., Honkura R., Nishimiya S., Ueno K., Mochizuki A., Tanimoto H., Tsugawa H., Otsuki Y., Ohashi Y., Efficient Promoter Cassettes for Enhanced Expression of Foregin Genes in Dicotyledonous and Monocotyledonous Plants, 10.1093/oxfordjournals.pcp.a028913
  19. L Moriau, Plant J, 19, 31 (1999)
  20. T Nagata, Inter Rev Cytol, 132, 1 (1992)
  21. U Pal, J Clin Invest, 106, 561 (2000)
  22. Pal U., Montgomery R. R., Lusitani D., Voet P., Weynants V., Malawista S. E., Lobet Y., Fikrig E., Inhibition of Borrelia burgdorferi-Tick Interactions In Vivo by Outer Surface Protein A Antibody, 10.4049/jimmunol.166.12.7398
  23. V Sambri, Arch Microbiol, 157, 205 (1992)
  24. Sambri Vittorio, Massaria Francesca, Ardizzoni Marina, Stefanelli Claudio, Cevenini Roberto, Glycoprotein patterns in Borrelia spp., 10.1016/s0934-8840(11)80365-8
  25. A Schiermeyer, Biotechnol Bioeng, 89, 848 (2005)
  26. TG Schwan, Borrelia burgdorferi Biochem Soc Transact, 31, 108 (2003)
  27. LH Sigal, New Engl J Med, 339, 216 (1998)
  28. AC Steere, New Engl J Med, 339, 209 (1998)
  29. Sutcliffe Iain C., Harrington Dean J., Pattern searches for the identification of putative lipoprotein genes in Gram-positive bacterial genomes, 10.1099/00221287-148-7-2065
  30. Thanassi Wendy Todaro, Schoen Robert T., The Lyme Disease Vaccine: Conception, Development, and Implementation, 10.7326/0003-4819-132-8-200004180-00009
  31. L Fits van der, Plant Mol Biol, 43, 495 (2000)
  32. JJ Weis, Infect Immun, 62, 4632 (1994)
  33. D Wessel, Anal Biochem, 138, 141 (1984)
  34. TA Willet, Proc Natl Acad Sci USA, 101, 1303 (2004)
  35. XF Yang, J Exp Med, 199, 641 (2004)
  36. A Yoder, Infect Immun, 71, 3894 (2003)
  37. R Zhao, Plant Sci, 149, 157 (1999)
Bibliographic reference Navarre, Catherine ; Delannoy, Mélanie ; Lefebvre, Benoit ; Nader, Joseph ; Vanham, Delphine ; et. al. Expression and secretion of recombinant outer-surface protein A from the Lyme disease agent, Borrelia burgdorferi, in Nicotiana tabacum suspension cells.. In: Transgenic research, Vol. 15, no. 3, p. 325-35 (2006)
Permanent URL http://hdl.handle.net/2078.1/10619