Alsteens, David
[UCL]
Dupres, Vincent
[UCL]
Dague, E.
[UCL]
Verbelen, Claire
[UCL]
André, Guillaume
[UCL]
Francius, Grégory
[UCL]
Dufrêne, Yves
[UCL]
Imaging the nanoscale distribution of specific chemical and biological sites on live cells is an important challenge in current life science research. In addition to imaging the surface topography of live cells, atomic force microscopy (AFM) is increasingly used to probe their chemical groups and biological receptors. In chemical force microscopy, AFM tips are modified with specific functional groups, thereby allowing investigators to probe chemical sites and their interactions on a scale of only ˜25 functional groups. In molecular recognition imaging, tips are functionalized with specific biomolecules, or samples labeled with immunogold particles, enabling researchers to localize specific receptors. Clearly, these nanoscale investigations provide new avenues in cellular biology and microbiology for elucidating the structure-function relationships of cell surfaces. In this chapter, we discuss the principles of these AFM modalities and their applications in life science research.


Bibliographic reference |
Alsteens, David ; Dupres, Vincent ; Dague, E. ; Verbelen, Claire ; André, Guillaume ; et. al. Imaging Chemical Groups and Molecular Recognition Sites on Live Cells Using AFM. In: B. Bhushan, H. Fuchs, Applied Scanning Probe Methods XIII, NanoScience and Technology, Springer-Verlag, ch. 10, : Berlin Heidelberg 2009, p.33-48 |
Permanent URL |
http://hdl.handle.net/2078.1/105568 |