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Inhibition of trypsin by the beta-amyloid protein precursor. A comparative study between transfected cells, human brain and cerebrospinal fluid.

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Delvaux, A. Van der Elst, L. Octave, Jean-Noël [UCL]

Soluble beta-amyloid protein precursors (beta-APPs) were studied in human brain and cerebrospinal fluid (CSF) after partial purification by ion exchange chromatography. Proteins were analysed in immunoblotting experiments using a monoclonal antibody directed against the N-terminal segment of the beta-APP 770, and by reverse enzymography. In the human brain and CSF, a protein which comigrates with the beta-APP 770 expressed by transfected CHO cells was able to inhibit trypsin.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1992
Journal information "FEBS letters" - Vol. 297, no. 1-2, p. 124-6 (1992)
Peer reviewed yes
issn 0014-5793
Publication status Publié
Affiliation UCL - MD/FSIO - Département de physiologie et pharmacologie
MESH Subject Amyloid beta-Protein Precursor - cerebrospinal fluid - isolation & purification - metabolism ; Animals ; Blotting, Western ; Brain - metabolism ; CHO Cells ; Cell Line ; Chromatography, DEAE-Cellulose ; Cricetinae ; Culture Media ; Electrophoresis, Polyacrylamide Gel ; Humans ; Transfection ; Trypsin Inhibitors
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Bibliographic reference Delvaux, A. ; Van der Elst, L. ; Octave, Jean-Noël. Inhibition of trypsin by the beta-amyloid protein precursor. A comparative study between transfected cells, human brain and cerebrospinal fluid.. In: FEBS letters, Vol. 297, no. 1-2, p. 124-6 (1992)
Permanent URL http://hdl.handle.net/2078.1/10010