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Involvement of TRPC in the abnormal calcium influx observed in dystrophic (mdx) mouse skeletal muscle fibers

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Vandebrouck, Clarisse [UCL] Martin, Dominique [UCL] Colson-Van Schoor, Monique [UCL] Debaix, Huguette [UCL] Gailly, Philippe [UCL]

Duchenne muscular dystrophy results from the lack of dystrophin, a cytoskeletal protein associated with the inner surface membrane, in skeletal muscle. The absence of dystrophin induces an abnormal increase of sarcolemmal calcium influx through cationic channels in adult skeletal muscle fibers from dystrophic (mdx) mice. We observed that the activity of these channels was increased after depletion of the stores of calcium with thapsigargin or caffeine. By analogy with the situation observed in nonexcitable cells, we therefore hypothesized that these store-operated channels could belong to the transient receptor potential channel (TRPC) family. We measured the expression of TRPC isoforms in normal and mdx adult skeletal muscles fibers, and among the seven known isoforms, five were detected (TRPC1, 2, 3, 4, and 6) by RT-PCR. Western blot analysis and immunocytochemistry of normal and mdx muscle fibers demonstrated the localization of TRPC1, 4, and 6 proteins at the plasma membrane. Therefore, an antisense strategy was used to repress these TRPC isoforms. In parallel with the repression of the TRPCs, we observed that the occurrence of calcium leak channels was decreased to one tenth of its control value (patch-clamp technique), showing the involvement of TRPC in the abnormal calcium influx observed in dystrophic fibers.

Type de document Article de périodique (Journal article) – Article de recherche
Type d'accès Accès libre
Année de publication 2002
Information sur le périodique "The Journal of Cell Biology" - Vol. 158, no. 6, p. 1089-1096 (2002)
Peer reviewed oui
Editeur Rockefeller University Press (New York)
issn 0021-9525
e-issn 1540-8140
Statut de la publication Publié
Affiliations UCL - MD/FSIO - Département de physiologie et pharmacologie
UCL - (SLuc) Service de neurologie
MESH Subject Animals ; Caffeine - pharmacology ; Calcium - analysis - metabolism ; Calcium Channels - chemistry - genetics - metabolism - physiology ; Cells, Cultured ; Dystrophin - genetics ; Enzyme Inhibitors - pharmacology ; Ion Channels - chemistry - genetics - metabolism ; Ion Transport ; Membrane Potentials ; Mice ; Mice, Inbred C57BL ; Mice, Inbred mdx ; Muscle Fibers - metabolism - physiology ; Muscle, Skeletal - metabolism - physiopathology ; Muscular Dystrophy, Animal - metabolism - physiopathology ; Patch-Clamp Techniques ; Sarcolemma - metabolism ; Sodium - metabolism ; TRPC Cation Channels ; Thapsigargin - pharmacology
Mots-clés Calcium channels ; Dystrophy ; Sarcoplasmic reticulum ; Capacitative entry ; TRP channel
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Référence bibliographique Vandebrouck, Clarisse ; Martin, Dominique ; Colson-Van Schoor, Monique ; Debaix, Huguette ; Gailly, Philippe. Involvement of TRPC in the abnormal calcium influx observed in dystrophic (mdx) mouse skeletal muscle fibers. In: The Journal of Cell Biology, Vol. 158, no. 6, p. 1089-1096 (2002)
Permalien http://hdl.handle.net/2078.1/9245