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Pulsatile activity and hatching of in vitro produced cow blastocysts: effects of serum supplementation.

Onglets principaux

van Heule, A Van Langendonckt, Anne [UCL] Donnay, Isabelle [UCL] Dessy, Franz [UCL] Massip, Alban [UCL]

The effect of serum added to a modified SOF medium on pulsatile activity and hatching of in vitro produced cow blastocysts was investigated by time-lapse cinematography. Embryos were generated from abattoir material and cultured in mSOF without serum or with 10% FCS added at 42h pi. Addition of serum significantly increases pulsatile activity before zona rupture and reduces the time of hatching. Pulsatile activity does not seem to be involved in the hatching process.

Type de document Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Année de publication 2001
Information sur le périodique "European journal of morphology" - Vol. 39, no. 2, p. 73-9 (2001)
Peer reviewed oui
issn 0924-3860
Statut de la publication Publié
Affiliations UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie
UCL - SC/BIOL - Département de biologie
MESH Subject Animals ; Blastocyst - drug effects - physiology ; Cattle - embryology ; Cell Culture Techniques - methods ; Culture Media ; Female ; Fetal Blood ; Humans ; Male ; Microscopy, Video ; Statistics as Topic ; Time Factors ; Zona Pellucida - physiology
Liens
Référence bibliographique van Heule, A ; Van Langendonckt, Anne ; Donnay, Isabelle ; Dessy, Franz ; Massip, Alban. Pulsatile activity and hatching of in vitro produced cow blastocysts: effects of serum supplementation.. In: European journal of morphology, Vol. 39, no. 2, p. 73-9 (2001)
Permalien http://hdl.handle.net/2078.1/9037