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Surface properties and cell adhesion onto allylamine-plasma and amine-plasma coated glass coverslips

Onglets principaux

Crespin, Marianne [FUNDP] Moreau, Nicolas [FUNDP] Masereel, Bernard [FUNDP] Feron, Olivier [UCL] Gallez, Bernard [UCL] Vander Borght, Thierry [UCL] Michiels, Carine [FUNDP] Lucas, Stephane [FUNDP]

Surface properties of nanoparticles to be used for radioimmunotherapy need to be optimized to allow antibody conjugation while ensuring biocompatibility. We aimed to investigate cell adhesion and proliferation onto different coatings to be used for nanoparticles. C, CH(x) or SiO(x) coatings deposited onto glass coverslips by magnetron deposition as well as nitrogen functionalized materials synthetized using different reactive sputtering conditions and PPAA (plasma polymerized allylamine) coating, were compared. Amine functionalization did increase hydrophilicity in all the materials tested. Biocompatibility was assessed by measuring cell viability, morphology, attachment, spreading, and pro-inflammatory cytokine secretion. The results show that C and CN(x) were the most biocompatible substrates while SiO(x) and SiO(x)N(y) were the most toxic materials. PPAA coatings displayed unexpectedly an intermediate biocompatibility. A correlation could be observed between wettability and cell proliferation except for C coated surface, indicating that more complex processes than hydrophilicity alone are taking place that affect cell functions.

Type de document Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Type d'accès Accès restreint
Année de publication 2011
Langue Anglais
Information sur le périodique "Journal of Materials Science: Materials in Medicine" - Vol. 22, no. 3, p. 671-682 (2011)
Peer reviewed oui
Editeur Springer New York LLC ((United States) New York)
issn 0957-4530
e-issn 1573-4838
Statut de la publication Publié
Affiliations UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique
UCL - SSS/LDRI - Louvain Drug Research Institute
UCL - SSS/IREC - Institut de recherche expérimentale et clinique
UCL - (MGD) Service de médecine nucléaire
UCL - (SLuc) Service de médecine nucléaire
MESH Subject Allylamine - chemistry ; Amines - chemistry ; Humans ; Inflammation ; Nanoparticles - chemistry ; Nanotechnology - methods ; Radioimmunotherapy - methods ; Surface Properties ; Antibodies - chemistry ; Biocompatible Materials - chemistry ; Cell Adhesion ; Cell Survival ; Coated Materials, Biocompatible - chemistry ; Cytokines - metabolism ; Endothelial Cells - cytology ; Glass
Liens
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Référence bibliographique Crespin, Marianne ; Moreau, Nicolas ; Masereel, Bernard ; Feron, Olivier ; Gallez, Bernard ; et. al. Surface properties and cell adhesion onto allylamine-plasma and amine-plasma coated glass coverslips. In: Journal of Materials Science: Materials in Medicine, Vol. 22, no. 3, p. 671-682 (2011)
Permalien http://hdl.handle.net/2078.1/89505