Ziegler, Andreas
Coulie, Pierre G.
[UCL]
Uchańska-Ziegler, Barbara
We will explain why major histocompatibility complex (MHC) molecules presenting peptides derived from tumour-associated antigens can be recognized not only by T cell receptors (TCR), but also by soluble proteins endowed with TCR-like reactivity. To understand how an antibody can display high affinity and specificity for a particular MHC:peptide complex, we have employed X-ray crystallography to determine the structure of a recombinant antibody, Hyb3, bound to human HLA-A1 molecules presenting the peptide EADPTGHSY that is derived from the tumour-associated antigen MAGE-Al. The results indicate that although Hyb3 recgonizes its target in a TCR-like diagonal binding mode, important differences between the two types of proteins exist that are probably due to the fact that TCR are part of a molecular assembly on the surface of effector cells, while antibodies such as Hyb3 have to carry out their function as individual molecules.
Référence bibliographique |
Ziegler, Andreas ; Coulie, Pierre G. ; Uchańska-Ziegler, Barbara. Monoclonal and recombinant antibodies with T cell receptor-like reactivity.. In: Recent Results in Cancer Research, Vol. 176, p. 229-241 (2007) |
Permalien |
http://hdl.handle.net/2078/88231 |