Menu utilisateur

Accès à distance ? S'identifier sur le proxy UCLouvain

New insight on water status in germinating Brassica napus seeds in relation to priming-improved germination

Onglets principaux

Télécharger
  • Open access
  • PDF
  • 4.13 M
Lechowska, Katarzyna [Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, AdamMickiewicz University, Pozna´n,] Kubala, Szymon [Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, AdamMickiewicz University, Pozna´n,] Wojtyla, Łukasz [Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, AdamMickiewicz University, Pozna´n] Nowaczyk, Grzegorz [NanoBioMedical Centre, Adam Mickiewicz University, Poznan] Quinet, Muriel [UCL] Lutts, Stanley [UCL] Garnczarska, Małgorzata [Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, AdamMickiewicz University]

Seed priming is a pre-sowing method successfully used to improve seed germination. Since water plays a crucial role in germination, the aim of this study was to investigate the relationship between better germination performances of osmoprimed Brassica napus seeds and seed water status during germination. To achieve this goal, a combination of different kinds of approaches was used, including nuclear magnetic resonance (NMR) spectroscopy, TEM, and SEM as well as semi-quantitative PCR (semi-qPCR). The results of this study showed that osmopriming enhanced the kinetics of water uptake and the total amount of absorbed water during both the early imbibition stage and in the later phases of seed germination. The spin–spin relaxation time (T2) measurement suggests that osmopriming causes faster water penetration into the seed and more efficient tissue hydration. Moreover, factors potentially affecting water relations in germinating primed seeds were also identified. It was shown that osmopriming (i) changes the microstructural features of the seed coat, e.g., leads to the formation of microcracks, (ii) alters the internal structure of the seed by the induction of additional void spaces in the seed, (iii) increases cotyledons cells vacuolization, and (iv) modifies the expression pattern of aquaporin genes.

Type de document Article de périodique (Journal article) – Article de recherche
Type d'accès Accès libre
Année de publication 2019
Langue Anglais
Information sur le périodique "International Journal of Molecular Sciences" - Vol. 20, no.3, p. 540 (2019)
Peer reviewed oui
Editeur MDPI AG
e-issn 1422-0067
Statut de la publication Publié
Affiliations UCL - SST/ELI/ELIA - Agronomy
Mots-clés Physical and Theoretical Chemistry ; Inorganic Chemistry ; Organic Chemistry ; Spectroscopy ; Molecular Biology ; Catalysis ; General Medicine ; Computer Science Applications
Liens
  1. Rakow, 3 (2004)
  2. Bewley (2013)
  3. Copeland (2012)
  4. SOUZA FRANCISCO H. DÜBBERN DE, MARCOS-FILHO JÚLIO, The seed coat as a modulator of seed-environment relationships in Fabaceae, 10.1590/s0100-84042001000400002
  5. Moïse Jaimie A., Han Shuyou, Gudynaitę-Savitch Loreta, Johnson Douglas A., Miki Brian L. A., Seed coats: Structure, development, composition, and biotechnology, 10.1079/ivp2005686
  6. Radchuk Volodymyr, Borisjuk Ljudmilla, Physical, metabolic and developmental functions of the seed coat, 10.3389/fpls.2014.00510
  7. Steinbrecher Tina, Leubner-Metzger Gerhard, The biomechanics of seed germination, 10.1093/jxb/erw428
  8. MA F., Cracks in the Palisade Cuticle of Soybean Seed Coats Correlate with their Permeability to Water, 10.1093/aob/mch133
  9. Wada Sugae, Kennedy James A., Reed Barbara M., Seed-coat anatomy and proanthocyanidins contribute to the dormancy of Rubus seed, 10.1016/j.scienta.2011.08.034
  10. Woodstock, J. Seed Technol., 12, 1 (1988)
  11. Heil, Lebensm. Wiss. Technol. Food Sci. Technol., 25, 280 (1992)
  12. Pietrzak L. N., Frégeau-Reid J., Chatson B., Blackwell B., Observations on water distribution in soybean seed during hydration processes using nuclear magnetic resonance imaging, 10.4141/p01-150
  13. Manz B., Water Uptake and Distribution in Germinating Tobacco Seeds Investigated in Vivo by Nuclear Magnetic Resonance Imaging, 10.1104/pp.105.061663
  14. KIKUCHI KAORI, KOIZUMI MIKA, ISHIDA NOBUAKI, KANO HIROMI, Water Uptake by Dry Beans Observed by Micro-magnetic Resonance Imaging, 10.1093/aob/mcl145
  15. Wojtyla Łukasz, Garnczarska Małgorzata, Zalewski Tomasz, Bednarski Waldemar, Ratajczak Lech, Jurga Stefan, A comparative study of water distribution, free radical production and activation of antioxidative metabolism in germinating pea seeds, 10.1016/j.jplph.2006.06.014
  16. Garnczarska Małgorzata, Zalewski Tomasz, Kempka Marek, Water uptake and distribution in germinating lupine seeds studied by magnetic resonance imaging and NMR spectroscopy, 10.1111/j.1399-3054.2007.00883.x
  17. Koizumi, Am. J. Biol. Life Sci., 2, 37 (2014)
  18. Munz Eberhard, Rolletschek Hardy, Oeltze-Jafra Steffen, Fuchs Johannes, Guendel André, Neuberger Thomas, Ortleb Stefan, Jakob Peter M., Borisjuk Ljudmilla, A functional imaging study of germinating oilseed rape seed, 10.1111/nph.14736
  19. Obroucheva N. V., Sinkevich I. A., Lityagina S. V., Novikova G. V., Water relations in germinating seeds, 10.1134/s102144371703013x
  20. De (2000)
  21. Sliwinska Elwira, Bassel George W., Bewley J. Derek, Germination of Arabidopsis thaliana seeds is not completed as a result of elongation of the radicle but of the adjacent transition zone and lower hypocotyl, 10.1093/jxb/erp203
  22. Weitbrecht Karin, Müller Kerstin, Leubner-Metzger Gerhard, First off the mark: early seed germination, 10.1093/jxb/err030
  23. Oracz Krystyna, Voegele Antje, Tarkowská Danuše, Jacquemoud Dominique, Turečková Veronika, Urbanová Terezie, Strnad Miroslav, Sliwinska Elwira, Leubner-Metzger Gerhard, Myrigalone A Inhibits Lepidium sativum Seed Germination by Interference with Gibberellin Metabolism and Apoplastic Superoxide Production Required for Embryo Extension Growth and Endosperm Rupture, 10.1093/pcp/pcr124
  24. Schuurmans Jolanda A.M.J., van Dongen Joost T., Rutjens Bas P.W., Boonman Alex, Pieterse Corné M.J., Borstlap Adrianus C., Members of the aquaporin family in the developing pea seed coat include representatives of the PIP, TIP, and NIP subfamilies, 10.1023/b:plan.0000019070.60954.77
  25. Vander Willigen Clare, Postaire Olivier, Tournaire-Roux Colette, Boursiac Yann, Maurel Christophe, Expression and Inhibition of Aquaporins in Germinating Arabidopsis Seeds , 10.1093/pcp/pcj094
  26. Liu Hong-Yan, Yu Xin, Cui Da-Yong, Sun Mei-Hao, Sun Wei-Ning, Tang Zhang-Cheng, Kwak Sang-Soo, Su Wei-Ai, The role of water channel proteins and nitric oxide signaling in rice seed germination, 10.1038/cr.2007.34
  27. Liu Chengwei, Fukumoto Tatsuya, Matsumoto Tadashi, Gena Patrizia, Frascaria Daniele, Kaneko Tomoyuki, Katsuhara Maki, Zhong Shihua, Sun Xiaoli, Zhu Yanming, Iwasaki Ikuko, Ding Xiaodong, Calamita Giuseppe, Kitagawa Yoshichika, Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination, 10.1016/j.plaphy.2012.11.018
  28. Ge F. W., Tao P., Zhang Y., Wang J. B., Characterization of AQP gene expressions in Brassica napus during seed germination and in response to abiotic stresses, 10.1007/s10535-013-0386-1
  29. Novikova Galina V., Tournaire-Roux Colette, Sinkevich Irina A., Lityagina Snejana V., Maurel Christophe, Obroucheva Natalie, Vacuolar biogenesis and aquaporin expression at early germination of broad bean seeds, 10.1016/j.plaphy.2014.05.014
  30. Maurel Christophe, Boursiac Yann, Luu Doan-Trung, Santoni Véronique, Shahzad Zaigham, Verdoucq Lionel, Aquaporins in Plants, 10.1152/physrev.00008.2015
  31. Lutts (2016)
  32. Chaumont F., Aquaporins Constitute a Large and Highly Divergent Protein Family in Maize, 10.1104/pp.125.3.1206
  33. Yaneff Agustín, Vitali Victoria, Amodeo Gabriela, PIP1 aquaporins: Intrinsic water channels or PIP2 aquaporin modulators?, 10.1016/j.febslet.2015.10.018
  34. Wojtyla Łukasz, Lechowska Katarzyna, Kubala Szymon, Garnczarska Małgorzata, Molecular processes induced in primed seeds—increasing the potential to stabilize crop yields under drought conditions, 10.1016/j.jplph.2016.04.008
  35. Kubala Szymon, Garnczarska Małgorzata, Wojtyla Łukasz, Clippe André, Kosmala Arkadiusz, Żmieńko Agnieszka, Lutts Stanley, Quinet Muriel, Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach, 10.1016/j.plantsci.2014.11.008
  36. Kubala Szymon, Wojtyla Łukasz, Quinet Muriel, Lechowska Katarzyna, Lutts Stanley, Garnczarska Małgorzata, Enhanced expression of the proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress, 10.1016/j.jplph.2015.04.009
  37. Yağmur, Afr. J. Biotechnol., 7, 2156 (2008)
  38. Galhaut Laurence, de Lespinay Alexis, Walker David J., Bernal Maria Pilar, Correal Enrique, Lutts Stanley, Seed Priming of Trifolium repens L. Improved Germination and Early Seedling Growth on Heavy Metal-Contaminated Soil, 10.1007/s11270-014-1905-1
  39. Gao Yong-Ping, Young Lester, Bonham-Smith Peta, Gusta Lawrence V., 10.1023/a:1006212216876
  40. Chen K., Fessehaie A., Arora R., Aquaporin expression during seed osmopriming and post-priming germination in spinach, 10.1007/s10535-012-0266-0
  41. Murley Margaret R., Seeds of the Cruciferae of Northeastern North America, 10.2307/2421948
  42. ZENG C.-L., Seed Coat Microsculpturing Changes during Seed Development in Diploid and Amphidiploid Brassica Species, 10.1093/aob/mch080
  43. Bove Jérôme, Jullien Marc, Grappin Philippe, 10.1186/gb-2001-3-1-reviews1002
  44. Obroucheva, 555 (1997)
  45. Soeda Y., Gene Expression Programs during Brassica oleracea Seed Maturation, Osmopriming, and Germination Are Indicators of Progression of the Germination Process and the Stress Tolerance Level, 10.1104/pp.104.051664
  46. Yacoubi Rafika, Job Claudette, Belghazi Maya, Chaibi Wided, Job Dominique, Toward Characterizing Seed Vigor in Alfalfa Through Proteomic Analysis of Germination and Priming, 10.1021/pr101274f
  47. Nagarajan Shantha, Nagarajan Shantha, Pandita V.K., Joshi D.K., Sinha J.P., Modi B.S., Characterization of water status in primed seeds of tomato (Lycopersicon esculentum Mill.) by sorption properties and NMR relaxation times, 10.1079/ssr2005200
  48. Parera, J. Am. Soc. Hortic. Sci., 116, 942 (1991)
  49. Osborne, 343 (2002)
  50. POWELL ALISON A., OLIVEIRA M. DE A., MATTHEWS S., The Role of Imbibition Damage in Determining the Vigour of White and Coloured Seed Lots of Dwarf French Beans (Phaseolus vulgaris), 10.1093/jxb/37.5.716
  51. Chachalis, Seed Sci. Technol., 28, 321 (2000)
  52. NAKAYAMA Norikazu, HASHIMOTO Shunji, SHIMADA Shinji, TAKAHASHI Motoki, KIM Yeong-Hoo, OYA Tetsuji, ARIHARA Joji, , 10.1626/jcs.73.323
  53. Isobe S, Effect of electric field on physical states of cell-associated water in germinating morning glory seeds observed by 1H-NMR, 10.1016/s0304-4165(98)00119-6
  54. Krishnan P., Joshi D.K., Nagarajan Shantha, Moharir A.V., Characterization of germinating and non-viable soybean seeds by nuclear magnetic resonance (NMR) spectroscopy, 10.1079/ssr2004189
  55. Vu, Pak. J. Bot., 46, 1847 (2014)
  56. Wolf, Scan. Electron Microsc., 3, 531 (1981)
  57. Liu Yongqing, Bino R. J., van der Burg W. J., Groot S. P. C., Hilhorst H. W. M., Effects of osmotic priming on dormancy and storability of tomato ( Lycopersicon esculentum Mill.) seeds, 10.1017/s0960258500003020
  58. Downie, Seed Sci. Res., 9, 117 (1999)
  59. Pandita, Indian J. Plant Physiol., 8, 249 (2003)
  60. Shijneva I. A., Novikova G. V., Obroucheva N. V., Aquaporins of tonoplast and plasmalemma in axial organs of germinating broad bean seeds, 10.1134/s1607672907020019
  61. Tiedemann Jens, Neubohn Birgit, Müntz Klaus, Different functions of vicilin and legumin are reflected in the histopattern of globulin mobilization during germination of vetch ( Vicia sativa L.), 10.1007/s004250000259
  62. Karaś Mieczysław, Activation of rape (Brassica napus L.) embryo during seed germination. V. The first zones of ultrastructural changes and their expansion, 10.5586/asbp.1987.009
  63. Bassel G. W., Stamm P., Mosca G., Barbier de Reuille P., Gibbs D. J., Winter R., Janka A., Holdsworth M. J., Smith R. S., Mechanical constraints imposed by 3D cellular geometry and arrangement modulate growth patterns in the Arabidopsis embryo, 10.1073/pnas.1404616111
  64. Bots M., PIP1 and PIP2 aquaporins are differentially expressed during tobacco anther and stigma development, 10.1093/jxb/eri009
  65. Joosen Ronny V. L., Kodde Jan, Willems Leo A. J., Ligterink Wilco, van der Plas Linus H. W., Hilhorst Henk W.M., germinator: a software package for high-throughput scoring and curve fitting of Arabidopsis seed germination : germinatorsoftware for Arabidopsis seed germination, 10.1111/j.1365-313x.2009.04116.x
  66. El-Kassaby, For. Sci., 54, 220 (2008)
  67. Meiboom S., Gill D., Modified Spin‐Echo Method for Measuring Nuclear Relaxation Times, 10.1063/1.1716296
  68. Provencher S. W., An eigenfunction expansion method for the analysis of exponential decay curves, 10.1063/1.432601
  69. Provencher Stephen W., Vogel Robert H., Information loss with transform methods in system identification: a new set of transforms with high information content, 10.1016/0025-5564(80)90040-1
  70. Borek Sławomir, Ratajczak Wiktoria, Ratajczak Lech, Ultrastructural and enzymatic research on the role of sucrose in mobilization of storage lipids in germinating yellow lupine seeds, 10.1016/j.plantsci.2005.09.011
  71. Karnovsky, J. Cell Biol., 27, 137 (1965)
  72. Spurr Arthur R., A low-viscosity epoxy resin embedding medium for electron microscopy, 10.1016/s0022-5320(69)90033-1
  73. Asif Mehar H., Dhawan Puneet, Nath Pravendra, A simple procedure for the isolation of high quality RNA from ripening banana fruit, 10.1007/bf02824018
Référence bibliographique Lechowska, Katarzyna ; Kubala, Szymon ; Wojtyla, Łukasz ; Nowaczyk, Grzegorz ; Quinet, Muriel ; et. al. New insight on water status in germinating Brassica napus seeds in relation to priming-improved germination. In: International Journal of Molecular Sciences, Vol. 20, no.3, p. 540 (2019)
Permalien http://hdl.handle.net/2078.1/212400