User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range alpha-amylase probe vectors.

Primary tabs

Hols, Pascal [UCL] Baulard, A Garmyn, D. Delplace, Brigitte [UCL] Hogan, S Delcour, Jean [UCL]

We have constructed two broad-host-range Gram+/Gram- probe vectors designed for the cloning of bacterial genetic expression and secretion signals. These vectors make use of a silent reporter gene encoding the mature alpha-amylase from Bacillus licheniformis whose reactivation can easily be monitored on iodine-stained starch plates. Shotgun cloning of Enterococcus faecalis DNA fragments allowed recovery of several cassettes directing transcription, translation of the reporter gene and secretion of alpha-amylase. Sequence analysis revealed, in each case, the presence of a putative promoter, ribosome-binding site and signal peptide similar to those described in other Gram+ bacteria.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Publication date 1992
Journal information "Gene" - Vol. 118, no. 1, p. 21-30 (1992)
Peer reviewed yes
issn 0378-1119
Publication status Publié
Affiliation UCL - SC/BIOL - Département de biologie
MESH Subject Amino Acid Sequence ; Bacillus - enzymology - genetics ; Base Sequence ; Chromosomes, Bacterial ; Cloning, Molecular - methods ; Enterococcus faecalis - genetics ; Escherichia coli - genetics ; Gene Expression Regulation, Bacterial ; Genetic Vectors - genetics ; Molecular Sequence Data ; Mutagenesis, Insertional - genetics ; Protein Biosynthesis ; Protein Conformation ; Protein Sorting Signals - genetics ; Recombinant Fusion Proteins - genetics ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Transcription, Genetic ; alpha-Amylase - genetics - secretion
Links
Bibliographic reference Hols, Pascal ; Baulard, A ; Garmyn, D. ; Delplace, Brigitte ; Hogan, S ; et. al. Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range alpha-amylase probe vectors.. In: Gene, Vol. 118, no. 1, p. 21-30 (1992)
Permanent URL http://hdl.handle.net/2078.1/9830