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Stable hepatitis C virus RNA detection by RT-PCR during four days storage.

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de Moreau de Gerbehaye, Anne-Isabelle Bodéus, Monique [UCL] Robert, Annie [UCL] Horsmans, Yves [UCL] Goubau, Patrick [UCL]

BACKGROUND: Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4 degrees C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples. METHODS: Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 - 25.4 degrees C) or at 4 degrees C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems). RESULTS: The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4 degrees C). CONCLUSIONS: We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4 degrees C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2002
Journal information "BMC Infectious Diseases" - Vol. 2, p. 22 (2002)
Publisher BioMed Central Ltd. (London)
issn 1471-2334
e-issn 1471-2334
Publication status Publié
Affiliations UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
UCL - MD/ESP - Ecole de santé publique
UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Service de microbiologie
UCL - (SLuc) Service d'hépato-gastro-entérologie
MESH Subject Adult ; Aged ; Blood Specimen Collection ; Female ; Hepacivirus - genetics - isolation & purification - physiology ; Humans ; Male ; Middle Aged ; RNA Stability ; RNA, Viral - analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Temperature ; Viral Load
Links
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Bibliographic reference de Moreau de Gerbehaye, Anne-Isabelle ; Bodéus, Monique ; Robert, Annie ; Horsmans, Yves ; Goubau, Patrick. Stable hepatitis C virus RNA detection by RT-PCR during four days storage.. In: BMC Infectious Diseases, Vol. 2, p. 22 (2002)
Permanent URL http://hdl.handle.net/2078.1/9265