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A new phenotype of Polymyxa betae in Arabidopsis thaliana

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Desoignies, Nicolas [UCL] Stocco, Céline [UCL] Bragard, Claude [UCL] Legrève, Anne [UCL]

The understanding of the molecular biology of Polymyxa betae, the protist vector of Beet necrotic yellow vein virus, remains limited because of the obligate nature of this root endoparasite and the limited data on the genome of Beta vulgaris, its most common host plant. The aim of this work was to assess the infection of P. betae in Arabidopsis thaliana in order to learn more about the P. betae genome and its interaction with the host. The susceptibility of a set of ecotypes of various origins to a monosporosorus and aviruliferous isolate of P. betae was analyzed in a series of bioassays conducted under controlled conditions. P. betae was detected in roots of A. thaliana using light microscopy and PCR. The infection severity was relatively low in this species compared with B. vulgaris, but the different stages of the life cycle were present. The phenotype of P. betae in A. thaliana root cells differed from the phenotype in B. vulgaris: the spore-forming phase was more prevalent in comparison with the sporangial phase, and the sporosori contained a lower number of spores. The compatible interaction between P. betae and A. thaliana obtained after the inoculation of zoospores and optimal conditions for the development of P. betae provide a new model system that can be used to improve the knowledge on the P. betae genome and on the mechanisms of the spore-forming phase of P. betae.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2011
Language Anglais
Journal information "European journal of plant pathology" - Vol. 131, no. 1, p. 27-38 (2011)
Peer reviewed yes
issn 0929-1873
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Links
  1. ABE Hideo, UI Tadao, Host range of Polymyxa betae Keskin strains in rhizomania-infested soils of sugar beet fields in Japan., 10.3186/jjphytopath.52.394
  2. ARCHIBALD JOHN M., KEELING PATRICK J., Actin and Ubiquitin Protein Sequences Support a Cercozoan/Foraminiferan Ancestry for the Plasmodiophorid Plant Pathogens, 10.1111/j.1550-7408.2004.tb00172.x
  3. Barr Donald J.S., Morphology and host range ofPolymyxa graminis, Polymyxa betae,andLigniera pilorumfrom Ontario and some other areas, 10.1080/07060667909501468
  4. BARR K. J., ASHER M. J. C., The host range of Polymyxa betae in Britain, 10.1111/j.1365-3059.1992.tb02317.x
  5. BARR K. J., ASHER M. J. C., LEWIS B. G., Resistance to Polymyxa betae in wild Beta species, 10.1111/j.1365-3059.1995.tb02781.x
  6. Braselton James P., Current Status of the Plasmodiophorids, 10.3109/10408419509113543
  7. Braselton, J. P. (2001). Plasmodiophoromycota. In: D. J. McLaughlin et al., (Eds.), The Mycota VII, Part A, systematics and evolution. Berlin, Germany: Springer-Verlag, pp. 81–91
  8. Brodmann David, Schuller Astrid, Ludwig-Müller Jutta, Aeschbacher Roger A., Wiemken Andres, Boller Thomas, Wingler Astrid, Induction of Trehalase inArabidopsisPlants Infected With the Trehalose-Producing PathogenPlasmodiophora brassicae, 10.1094/mpmi.2002.15.7.693
  9. Bulman Simon R., Kühn Stefanie F., Marshall John W., Schnepf Eberhard, A Phylogenetic Analysis of the SSU rRNA from Members of the Plasmodiophorida and Phagomyxida, 10.1078/1434-4610-00042
  10. Bulman Simon, Siemens Johannes, Ridgway Hayley J., Eady Colin, Conner Anthony J., Identification of genes from the obligate intracellular plant pathogen,Plasmodiophora brassicae, 10.1111/j.1574-6968.2006.00466.x
  11. Crutzen F., Kreit M., Bragard C., The beet virus Q coat protein readthrough domain is longer than previously reported, with two transmembrane domains, 10.1099/vir.0.008029-0
  12. Crutzen F., Mehrvar M., Gilmer D., Bragard C., A full-length infectious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves, 10.1099/vir.0.014548-0
  13. Delfosse, P., Reddy, S. A., Legrève, A., Thirumala Devi, K., Abdurahman, M. D., Maraite, H., et al. (2000). Serological methods for detection of Polymyxa graminis, an obligate root parasite and vector of plant viruses. Virology, 90, 537–545.
  14. Desoignies N., Eickermann M., Delfosse P., Kremer F., Godart N., Hoffmann L., Legrève A., First Report of Plasmodiophora brassicae on Rapeseed in the Grand Duchy of Luxembourg, 10.1094/pdis-93-11-1220b
  15. FUJISAWA Ichiro, SUGIMOTO Toshiya, Transmission of Beet Necrotic Yellow Vein Virus by Polymyxa betae, 10.3186/jjphytopath.43.583
  16. Grishkan I., Korol A. B., Nevo E., Wasser S. P., Ecological stress and sex evolution in soil microfungi, 10.1098/rspb.2002.2194
  17. Keskin, B. (1964). Polymyxa betae n.sp., ein Parasit in den Wurzeln von Beta vulgaris Tournefort, besonders während der Jugendentwicklung der Zuckerrübe. Archives of Microbiology, 49, 348–374.
  18. Kobelt Peter, Siemens Johannes, Sacristán María Dolores, Histological characterisation of the incompatible interaction between Arabidopsis thaliana and the obligate biotrophic pathogen Plasmodiophora brassicae, 10.1017/s0953756299001781
  19. Koch E., Cox R., Williams P. H., Infection of Arabidopsis thaliana by Plasmodiophora brassicae, 10.1111/j.1439-0434.1991.tb00100.x
  20. Lee C.-W., Efetova M., Engelmann J. C, Kramell R., Wasternack C., Ludwig-Muller J., Hedrich R., Deeken R., Agrobacterium tumefaciens Promotes Tumor Induction by Modulating Pathogen Defense in Arabidopsis thaliana, 10.1105/tpc.108.064576
  21. Legrève Anne, Delfosse Philippe, Vanpee Brigitte, Goffin André, Maraite Henri, 10.1023/a:1008612903927
  22. Legrève Anne, Vanpee Brigitte, Delfosse Philippe, Maraite Henri, 10.1023/a:1008784823899
  23. Legrève Anne, Delfosse Philippe, Maraite Henri, Phylogenetic analysis of Polymyxa species based on nuclear 5.8S and internal transcribed spacers ribosomal DNA sequences, 10.1017/s0953756201005391
  24. Legrève, A., Delfosse, P., Van Hese, V., Bragard, C., & Maraite, H. (2003). Broad-spectrum detection of Polymyxa species and form species by polymerase chain reaction. In: C. M. Rush & U. Merz (Eds). Proceedings of the fifth Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 22–25 July 2002, IPS, Zurich, Switzerland. pp. 40–43 Denver, USA: American Society of Sugar Beet Technologists)
  25. Legrève, A., Schmit, J. F., Bragard, C., & Maraite H. (2005). The role of climate and Alternative hosts in the epidemiology of rhizomania. In: C. M. Rush (Ed.), Proceedings of the sixth Symposium of the International Working Group on Plant Viruses with Fungal Vectors, 5–7 September 2005, Bologna Italy. (pp. 125–128). Denver, USA: American Society of Sugar Beet Technologists).
  26. Littlefield L. J., Whallon J. H., Doss P. J., Hassan Z. M., Postinfection Development of Polymyxa graminis in Roots of Triticum aestivum, 10.2307/3761328
  27. McGrann Graham R. D., Martin Lydia D., Kingsnorth Crawford S., Asher Michael J. C., Adams Michael J., Mutasa-Göttgens Effie S., Screening for genetic elements involved in the nonhost response of sugar beet to the plasmodiophorid cereal root parasite Polymyxa graminis by representational difference analysis, 10.1007/s10327-007-0024-x
  28. McGrann Graham R. D., Townsend Belinda J., Antoniw John F., Asher Michael J. C., Mutasa-Göttgens Effie S., Barley elicits a similar early basal defence response during host and non-host interactions with Polymyxa root parasites, 10.1007/s10658-008-9332-z
  29. Mutasa E.S., Ward E., Adams M.J., Collier C.R., Chwarszczynska D.M., Asher M.J.C., A sensitive DNA probe for the detection of Polymyxa betae in sugar beet roots, 10.1006/pmpp.1993.1066
  30. Mutasa-Gottgens E.S., Chwarszczynska D.M., Halsey K., Asher M.J.C., Specific polyclonal antibodies for the obligate plant parasite Polymyxa- a targeted recombinant DNA approach, 10.1046/j.1365-3059.2000.00446.x
  31. Obermeier, C. (1998). New Diagnostic tools for detection of polymyxa betae in sugar beet roots. PhD Thesis, Technical University Braunschweig, Germany.
  32. Peltier, C., Hleibieh, K., Thiel, H., Klein, E., Bragard, C., & Gilmer, D. (2008). Molecular biology of the beet necrotic yellow vein virus. Plant Viruses, 2, 14–24.
  33. Pferdmenges Friederike, Korf Helmut, Varrelmann Mark, Identification of rhizomania-infected soil in Europe able to overcome Rz1 resistance in sugar beet and comparison with other resistance-breaking soils from different geographic origins, 10.1007/s10658-008-9388-9
  34. Rush Charles M., Liu H.-Y., Lewellen R. T., Acosta-Leal R., The Continuing Saga of Rhizomania of Sugar Beets in the United States, 10.1094/pd-90-0004
  35. Ruzin, S. E. (1999). Plant microtechnique and microscopy. Oxford: Oxford University Press.
  36. Siemens Johannes, Keller Ingo, Sarx Johannes, Kunz Sabine, Schuller Astrid, Nagel Wolfgang, Schmülling Thomas, Parniske Martin, Ludwig-Müller Jutta, Transcriptome Analysis ofArabidopsisClubroots Indicate a Key Role for Cytokinins in Disease Development, 10.1094/mpmi-19-0480
  37. Thatcher Louise F., Manners John M., Kazan Kemal, Fusarium oxysporumhijacks COI1-mediated jasmonate signaling to promote disease development in Arabidopsis, 10.1111/j.1365-313x.2009.03831.x
  38. Ward E., Adams M.J., Analysis of ribosomal DNA sequences of Polymyxa species and related fungi and the development of genus- and species-specific PCR primers, 10.1017/s0953756297005881
  39. WISLER G. C., Beet Necrotic Yellow Vein Virus and Its Relationship to Eight Sugar Beet Furo-like Viruses from the United States, 10.1094/pd-78-0995
  40. Yuan Jiazheng, Zhu Mengxia, Lightfoot David A, Iqbal M Javed, Yang Jack Y, Meksem Khalid, In silico comparison of transcript abundances during Arabidopsis thaliana and Glycine max resistance to Fusarium virguliforme, 10.1186/1471-2164-9-s2-s6
Bibliographic reference Desoignies, Nicolas ; Stocco, Céline ; Bragard, Claude ; Legrève, Anne. A new phenotype of Polymyxa betae in Arabidopsis thaliana. In: European journal of plant pathology, Vol. 131, no. 1, p. 27-38 (2011)
Permanent URL http://hdl.handle.net/2078.1/91725