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Development of biocompatible oxygen-permeable films holding paramagnetic carbon particles: evaluation of their performance and stability in EPR oximetry.

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Jia, He [UCL] Beghein, Nelson [UCL] Ceroke, P Clarkson, R B Swartz, H M Gallez, Bernard [UCL]

EPR oximetry using paramagnetic particles relies on the measurement of the EPR linewidth, which is directly related to the pO2. It was previously found that some of the paramagnetic materials with optimal EPR spectroscopic properties in vitro may lose their responsiveness to oxygen in tissues (change of the calibration curve of the EPR linewidth as a function of the pO2). We hypothesized that coating paramagnetic particle materials could improve the stability of response, as well as the biocompatibility. In this study, very thin films holding paramagnetic materials were prepared with different biopolymers (cellulose acetate, cellulose triacetate, cellulose nitrate, silicone, and polyurethane) that already are accepted for clinical applications. Their performance was evaluated in EPR oximetry by measuring the stability of the calibration curves (EPR linewidth as a function of pO2) after a prolonged period in an aqueous environment (1 week in saline) or in vivo (implantation for 3 weeks under the skin of mice). We found that one type of silicone film was able to stabilize the responsiveness of an intrinsically unstable carbon material (a wood char).

Document type Article de périodique (Journal article) – Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Access type Accès restreint
Publication date 2001
Journal information "Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine" - Vol. 46, no. 3, p. 610-4 (2001)
Peer reviewed yes
issn 0740-3194
Publication status Publié
Affiliations UCL - MD/FARM - Ecole de pharmacie
UCL - MD/MINT - Département de médecine interne
MESH Subject Animals ; Biocompatible Materials ; Biopolymers ; Calibration ; Charcoal ; Electron Spin Resonance Spectroscopy - instrumentation ; Mice ; Mice, Inbred Strains ; Oximetry - instrumentation ; Prostheses and Implants ; Sensitivity and Specificity
Links
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Bibliographic reference Jia, He ; Beghein, Nelson ; Ceroke, P ; Clarkson, R B ; Swartz, H M ; et. al. Development of biocompatible oxygen-permeable films holding paramagnetic carbon particles: evaluation of their performance and stability in EPR oximetry.. In: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, Vol. 46, no. 3, p. 610-4 (2001)
Permanent URL http://hdl.handle.net/2078.1/8940