Combined cross-linked enzyme aggregates from versatile peroxidase and glucose oxidase: Production, partial characterization and application for the elimination of endocrine disruptors

Taboada-Puig, R. [University of Santiago de Compostela] Junghanns, Charles [UCL] Demarche, Philippe [UCL] Moreira, M.T. [University of Santiago de Compostela] Feijoo, G. [University of Santiago de Compostela] Lema, J.M. [University of Santiago de Compostela] Agathos, Spiros N. [UCL] et al.[show all]

Versatile peroxidase (VP) from Bjerkandera adusta was insolubilized in the form of cross-linked enzyme aggregates (CLEA(R)s). Of the initially applied activity 67% was recovered as CLEA(R)s. Co-aggregation of VP with glucose oxidase from Aspergillus niger led to an increased activity recovery of 89%. The combined CLEA(R)s showed higher stability against H(2)O(2) and exerted VP activity upon glucose addition. The elimination of the endocrine disrupting chemicals bisphenol A, nonylphenol, triclosan, 17 alpha-ethinylestradiol and the hormone 17 beta-estradiol (10 mg L(-1) each) and the removal of their estrogenic activity by combined CLEA(R)s were tested in batch experiments. Within 10 min, the combined CLEA(R)s were able to remove all the endocrine disruptors except triclosan (residual concentration 74%). The removal of the estrogenic activity was higher than 55% for all compounds, except triclosan. A membrane reactor continuously operated with combined CLEA(R)s could almost completely remove bisphenol A (10 mg L(-1)) for 43 h. (C) 2011 Elsevier Ltd. All rights reserved.