User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

YscP, a Yersinia protein required for Yop secretion that is surface exposed, and released in low Ca2+.

Primary tabs

Stainier, I. Bleves, S Josenhans, C Karmani, Linda [UCL] Kerbourch, C Lambermont, Isabelle [UCL] Cornelis, Guy

The Yersinia Ysc apparatus is made of more than 20 proteins, 11 of which have homologues in many type III systems. Here, we characterize YscP from Yersinia enterocolitica. This 515-residue protein has a high proline content, a large tandem repetition and a slow migration in SDS-PAGE. Unlike the products of neighbouring genes, it has a counterpart only in Pseudomonas aeruginosa and it varies even between Yersinia Ysc machineries. An yscPDelta97-465 mutant was unable to secrete any Yop, even under conditions overcoming feedback inhibition of Yop synthesis. Interestingly, a cloned yscPDelta57-324 from Yersinia pestis introduced in the yscPDelta97-465 mutant can sustain a significant Yop secretion and thus partially complemented the mutation. This explains the leaky phenotype observed with the yscP mutant of Y. pestis. In accordance with this secretion deficiency, YscP is required for the delivery of Yop effectors into macrophages. Mechanical shearing, immunolabelling and electron microscopy experiments showed that YscP is exposed at the bacterial surface when bacteria are incubated at 37 degrees C in the presence of Ca2+ and thus do not secrete Yops. At 37 degrees C, when Ca2+ ions are chelated, YscP is released like a Yop protein. We conclude that YscP is a part of the Ysc injectisome which is localized at the bacterial surface and is destabilized by Ca2+ chelation.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 2000
Journal information "Molecular microbiology" - Vol. 37, no. 5, p. 1005-18 (2000)
Peer reviewed yes
issn 0950-382X
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Bacterial Outer Membrane Proteins - metabolism ; Bacterial Proteins - genetics - metabolism ; Biological Transport ; Calcium - metabolism ; Cell Membrane - metabolism ; Cells, Cultured ; Eukaryotic Cells - microbiology ; Macrophages - cytology - metabolism - microbiology ; Membrane Proteins ; Yersinia enterocolitica - metabolism ; Yersinia pestis - metabolism
Links
  1. Cornelis G.R., Microbiol Mol Biol Rev, 62, 1315 (1998)
  2. Lambert de Rouvroit C.L., Mol Microbiol, 6, 395 (1992)
  3. Cornelis G.R., J Bacteriol, 171, 254 (1989)
  4. Pettersson J.R., Science, 273, 1231 (1996)
  5. Payne P.L., J Bacteriol, 180, 3882 (1998)
  6. Payne P.L., J Bacteriol, 181, 2852 (1999)
  7. Day J. B., Plano G. V., The Yersinia pestis YscY Protein Directly Binds YscX, a Secreted Component of the Type III Secretion Machinery, 10.1128/jb.182.7.1834-1843.2000
  8. Koster Margot, Bitter Wilbert, de Cock Hans, Allaoui Abdelmounaaıamp;;uml;m, Cornelis Guy R., Tommassen Jan, The outer membrane component, YscC, of the Yop secretion machinery ofYersinia enterocoliticaforms a ring-shaped multimeric complex, 10.1046/j.1365-2958.1997.6141981.x
  9. Allaoui A., J Bacteriol, 177, 4230 (1995)
  10. Michiels T., J Bacteriol, 173, 4994 (1991)
  11. Plano G.V., J Bacteriol, 177, 3843 (1995)
  12. Bergman T., J Bacteriol, 176, 2619 (1994)
  13. Fields K.A., J Bacteriol, 176, 569 (1994)
  14. Allaoui A., J Bacteriol, 176, 4534 (1994)
  15. Plano G.V., J Bacteriol, 173, 7293 (1991)
  16. Plano G.V., J Bacteriol, 175, 3536 (1993)
  17. Woestyn S., J Bacteriol, 176, 1561 (1994)
  18. Cornelis G.R., Microb Pathog, 2, 367 (1987)
  19. Boland A., Infect Immun, 66, 1878 (1998)
  20. Iriarte Maite, Sory Marie-Paule, Boland Anne, Boyd Aoife P., Mills Scott D., Lambermont Isabelle, Cornelis Guy R., TyeA, a protein involved in control of Yop release and in translocation of Yersinia Yop effectors, 10.1093/emboj/17.7.1907
  21. Kubori T., Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System, 10.1126/science.280.5363.602
  22. Blocker Ariel, Gounon Pierre, Larquet Eric, Niebuhr Kirsten, Cabiaux Véronique, Parsot Claude, Sansonetti Philippe, The Tripartite Type III Secreton ofShigella flexneriInserts Ipab and Ipac into Host Membranes, 10.1083/jcb.147.3.683
  23. Kapatral V., Mol Microbiol, 17, 49 (1995)
  24. Pettersson Jonas, Holmstrom Anna, Hill Jim, Leary Sophie, Frithz-Lindsten Elisabet, von Euler-Matell Anne, Carlsson Eva, Titball Richard, Forsberg Ake, Wolf-Watz Hans, The V-antigen of Yersinia is surface exposed before target cell contact and involved in virulence protein translocation, 10.1046/j.1365-2958.1999.01408.x
  25. Forsberg A., Mol Microbiol, 5, 977 (1991)
  26. Sarker M.R., J Bacteriol, 180, 1207 (1998)
  27. Roine E., Wei W., Yuan J., Nurmiaho-Lassila E.-L., Kalkkinen N., Romantschuk M., He S. Y., Hrp pilus: An hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000, 10.1073/pnas.94.7.3459
  28. Van Gijsegem Frederique, Vasse Jacques, Camus Jean-Christophe, Marenda Marc, Boucher Christian, Ralstonia solanacearum produces Hrp-dependent pili that are required for PopA secretion but not for attachment of bacteria to plant cells, 10.1046/j.1365-2958.2000.01851.x
  29. Wei W., Plovanich-Jones A., Deng W.-L., Jin Q.-L., Collmer A., Huang H.-C., He S. Y., The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato, 10.1073/pnas.040570097
  30. Knutton S., A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells, 10.1093/emboj/17.8.2166
  31. Hartland Elizabeth L., Daniell Sarah J., Delahay Robin M., Neves Bianca C., Wallis Tim, Shaw Robert K, Hale Christine, Knutton Stuart, Frankel Gad, The type III protein translocation system of enteropathogenic Escherichia coli involves EspA-EspB protein interactions : EspA-EspB protein interactions, 10.1046/j.1365-2958.2000.01814.x
  32. Sory M.P., Proc Natl Acad Sci USA, 92, 11998 (1995)
  33. Kaniga Koné, Delor Isabelle, Cornelis Guy R., A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica, 10.1016/0378-1119(91)90599-7
  34. Iriarte M., J Bacteriol, 177, 2299 (1995)
  35. Miller V.L., J Bacteriol, 170, 2575 (1988)
  36. Bachmann B.J., Microbiol Rev, 54, 130 (1990)
  37. Kunkel T.A., Methods Enzymol, 154, 357 (1987)
  38. Neyt C., J Bacteriol, 179, 612 (1997)
  39. Sambrook J., Molecular Cloning: a Laboratory Manual. (1989)
  40. Altshul S.F., J Bacteriol, 215, 403 (1990)
  41. Laemmli U.K., Nature, 227, 680 (1970)
  42. Wray W., Anal Biochem, 118, 197 (1981)
  43. Towbin H., Proc Natl Acad Sci USA, 76, 4350 (1979)
  44. Michiels T., Infect Immun, 58, 2840 (1990)
  45. Aizawa S.-C., J Bacteriol, 161, 836 (1985)
  46. Sory M.P., Mol Microbiol, 14, 583 (1994)
  47. Allaoui A., Mol Microbiol, 18, 343 (1995)
  48. Boland A., EMBO J, 15, 5191 (1996)
  49. Forsberg A., Mol Microbiol, 2, 121 (1988)
  50. Portnoy D.A., Infect Immun, 31, 775 (1981)
Bibliographic reference Stainier, I. ; Bleves, S ; Josenhans, C ; Karmani, Linda ; Kerbourch, C ; et. al. YscP, a Yersinia protein required for Yop secretion that is surface exposed, and released in low Ca2+.. In: Molecular microbiology, Vol. 37, no. 5, p. 1005-18 (2000)
Permanent URL http://hdl.handle.net/2078.1/8627