Mandruzzato, Susanna
Stroobant, Vincent
[UCL]
Demotte, Nathalie
[UCL]
van der Bruggen, Pierre
[UCL]
A CTL clone that recognizes autologous tumor cells was previously isolated from the blood of a head-and-neck cancer patient. The Ag was identified as peptide FPSDSWCYF presented by autologous HLA-B*3503 molecules. This peptide was encoded by a mutated CASP-8 gene, which is implicated in the triggering of apoptosis. Here, we show that this CTL clone, which expresses a single TCR, also recognizes two unrelated peptides on allogeneic HLA-B*3501 molecules. One peptide, HIPDVITY, is encoded by squalene synthase, and the other one, QFADVIVLF, is encoded by 2-hydroxyphytanoyl-CoA lyase. Both genes are expressed ubiquitously. These antigenic peptides are processed and presented by HLA-B*3501 cells. The two HLA-B35 alleles are closely related. Our results might reinforce the notion that the recognition of allogeneic HLA molecules depends on the presence in their groove of a limited number of peptides processed from ubiquitous proteins.
Bibliographic reference |
Mandruzzato, Susanna ; Stroobant, Vincent ; Demotte, Nathalie ; van der Bruggen, Pierre. A human CTL recognizes a caspase-8-derived peptide on autologous HLA-B*3503 molecules and two unrelated peptides on allogeneic HLA-B*3501 molecules. In: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, no. 8, p. 4130-4 (2000) |
Permanent URL |
http://hdl.handle.net/2078.1/8495 |