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Apoptosis induced by growth factor withdrawal in fibroblasts overproducing fructose 2,6-bisphosphate.

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Durante, Paula [UCL] Gueuning, Marie-Agnès [UCL] Darville, Martine I. [UCL] Hue, Louis [UCL] Rousseau, Guy [UCL]

Fructose 2,6-bisphosphate is a potent endogenous stimulator of glycolysis. A high aerobic glycolytic rate often correlates with increased cell proliferation. To investigate this relationship, we have produced clonal cell lines of Rat-1 fibroblasts that stably express transgenes coding for 6-phosphofructo-2-kinase, which catalyzes the synthesis of fructose 2,6-bisphosphate, or for fructose 2,6-bisphosphatase, which catalyzes its degradation. While serum deprivation in culture reduced the growth rate of control cells, it caused apoptosis in cells overproducing fructose 2,6-bisphosphate. Apoptosis was inhibited by 5-amino-4-imidazolecarboxamide riboside, suggesting that 5'-AMP-activated protein kinase interferes with this phenomenon.

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Bibliographic reference Durante, Paula ; Gueuning, Marie-Agnès ; Darville, Martine I. ; Hue, Louis ; Rousseau, Guy. Apoptosis induced by growth factor withdrawal in fibroblasts overproducing fructose 2,6-bisphosphate.. In: FEBS Letters, Vol. 448, no. 2-3, p. 239-243 (1999)
Permanent URL http://hdl.handle.net/2078.1/8262