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Identification of MAGE-3 epitopes presented by HLA-DR molecules to CD4(+) T lymphocytes.

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Chaux, Pascal [UCL] Vantomme, Valérie [UCL] Stroobant, Vincent [UCL] Thielemans, Kris Corthals, Jurgen Luiten, Rosalie [UCL] Boon, Thierry [UCL] van der Bruggen, Pierre [UCL]

MAGE-type genes are expressed by many tumors of different histological types and not by normal cells, except for male germline cells, which do not express major histocompatibility complex (MHC) molecules. Therefore, the antigens encoded by MAGE-type genes are strictly tumor specific and common to many tumors. We describe here the identification of the first MAGE-encoded epitopes presented by histocompatibility leukocyte antigen (HLA) class II molecules to CD4(+) T lymphocytes. Monocyte-derived dendritic cells were loaded with a MAGE-3 recombinant protein and used to stimulate autologous CD4(+) T cells. We isolated CD4(+) T cell clones that recognized two different MAGE-3 epitopes, MAGE-3114-127 and MAGE-3121-134, both presented by the HLA-DR13 molecule, which is expressed in 20% of Caucasians. The second epitope is also encoded by MAGE-1, -2, and -6. Our procedure should be applicable to other proteins for the identification of new tumor-specific antigens presented by HLA class II molecules. The knowledge of such antigens will be useful for evaluation of the immune response of cancer patients immunized with proteins or with recombinant viruses carrying entire genes coding for tumor antigens. The use of antigenic peptides presented by class II in addition to peptides presented by class I may also improve the efficacy of therapeutic antitumor vaccination.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 1999
Journal information "The Journal of Experimental Medicine" - Vol. 189, no. 5, p. 767-777 (1999)
Peer reviewed yes
Publisher Rockefeller University Press (New York)
issn 0022-1007
e-issn 1540-9538
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Amino Acid Sequence ; Antigen Presentation ; Antigens, Neoplasm - immunology ; CD4-Positive T-Lymphocytes - immunology ; Clone Cells ; Coculture Techniques ; Dendritic Cells - immunology ; Epitopes - immunology ; HLA-DR Antigens - immunology ; Humans ; Male ; Melanoma - immunology ; Molecular Sequence Data ; Neoplasm Proteins - immunology ; Peptides - immunology
Keywords Human ; Invariant chain ; Peptide ; Tumor ; Histocompatibility leukocyte antigen class II
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Bibliographic reference Chaux, Pascal ; Vantomme, Valérie ; Stroobant, Vincent ; Thielemans, Kris ; Corthals, Jurgen ; et. al. Identification of MAGE-3 epitopes presented by HLA-DR molecules to CD4(+) T lymphocytes.. In: The Journal of Experimental Medicine, Vol. 189, no. 5, p. 767-777 (1999)
Permanent URL http://hdl.handle.net/2078.1/8239