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Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities

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Chaumont, François [University California, San Diego] Loomis, WF [] Chrispeels, MJ []

The rd28 gene of Arabidopsis thaliana encodes a water channel protein, or aquaporin, of the plasma membrane. A construct in which transcription of the rd28 cDNA is controlled by the Dictyostelium actin15 promoter was transformed into Dictyostelium discoideum cells, Transformants contained RD28 protein in their plasma membranes. When shifted to a low-osmotic-strength buffer, cells expressing rd28 swelled rapidly and burst, indicating that the plant aquaporin allowed rapid water entry in the amoebae, The rate of osmotic lysis was a function of the osmotic pressure of the buffer, We also selected transformants in which the expression of the rd28 cDNA is driven by the promoter of the prespore cotB gene, These transformants accumulated rd28 mRNA uniquely in prespore cells, In low-osmotic-strength buffer, the cotB::rd28 cells aggregated and formed normally proportioned slugs but failed to form normal fruiting bodies, The number of spores was reduced 20-fold, and the stalks of the fruiting bodies mere abnormally short. The consequences of expressing RD28 in prespore cells could be partially overcome by increasing the osmolarity of the medium, Under these conditions, the cotBr::rd28 cells formed fruiting bodies of more normal appearance, and the number of viable sports increased slightly. Because prespore cells hare to shrink and dehydrate to form spores, it was not unexpected that expression of an aquaporin would disrupt this process, but it was surprising to find that stalk differentiation was also affected by expression of rd28 in prespore cells, It appears that, osmotic stress on prespore cells alters their ability to signal terminal differentiation in prestalk cells, The results provide independent confirmation that plant aquaporins can function in the cells of other organisms, and that D. discoideum can be used to study the properties of these water channels.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1997
Language Anglais
Journal information "Proceedings of the National academy of sciences of the United States of America" - Vol. 94, no. 12, p. 6202-6209 (1997)
Peer reviewed yes
Publisher National academy of sciences ((United States) Washington, D.C.)
issn 0027-8424
e-issn 1091-6490
Publication status Publié
Affiliations University California, San Diego - Department of Biology
UCL - Autre
Links
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Bibliographic reference Chaumont, François ; Loomis, WF ; Chrispeels, MJ ; et. al. Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities. In: Proceedings of the National academy of sciences of the United States of America, Vol. 94, no. 12, p. 6202-6209 (1997)
Permanent URL http://hdl.handle.net/2078.1/70290