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Modulation of signalling nuclear factor-kappa B activation pathway by polyphenols in human intestinal Caco-2 cells

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Romier, Beatrice Van De Walle, Jacqueline [UCL] During, Alexandrine Larondelle, Yvan [UCL] Schneider, Yves-Jacques [UCL]

Recent Studies support beneficial effects of polyphenols in various chronic inflammatory diseases, for example, the inflammatory bowel diseases. Inhibition of NF-kappa B activation by polyphenols could explain part of their anti-inflammatory properties, but few data are available on the intestine. The purpose of the present Study was thus to investigate the effects of some polyphenols on NF-kappa B activation using human intestinal Caco-2 cells. Effects of standard polyphenols (50 mu mol/l) were studied on different cellular events associated with NF-kappa B activation: (i) NF-kappa B activity using cells transiently transfected with a NF-kappa B-luciferase construct and stimulated by inflammatory agents (IL-1 beta, TNF-alpha or lipopolysaccharides (LPS)); (ii) phosphorylation of the inhibitor of kappa B (I kappa B-alpha) analysed by Western blot; (iii) secretion of IL-8 quantified by ELISA assay. Results showed that chrysin and ellagic acid inhibited NF-kappa B activity, whereas genistein and resveratrol increased it. These effects were independent of the nature of the inducer, indicating that polyphenols may modulate NF-kappa B activation by acting on a common event to the cytokine- and LPS-mediated cascades. Chrysin strongly reduced (2.5-fold) IL-1 beta-induced I kappa B-alpha phosphorylation, whereas ellagic acid increased it (1.7-fold). Ellagic acid, genistein and epigallocatechin.-allate reduced (4- to 8-fold) IL-1 beta-induced IL-8 secretion, while resveratrol promoted (1.7-fold) the secretion. Chrysin also diminished IL-8 secretion by 1.6-fold (but P > 0.05). The data indicate that polyphenols can modulate the NF-kappa B activation pathway in the intestine. Chrysin could block NF-kappa B activation via the inhibition of I kappa B-alpha phosphorylation. The other molecular targets of the active polyphenols are still to be identified.

Document type Communication à un colloque (Conference Paper) – Présentation orale avec comité de sélection
Access type Accès restreint
Publication date 2008
Language Anglais
Conference "23rd International Conference on Polyphenols", Winnipeg(Canada) (Aug, 2006)
Journal information "The British Journal of Nutrition : an international journal of nutritional science" - Vol. 100, no. 3, p. 542-551 (2008)
Peer reviewed yes
issn 0007-1145
e-issn 1475-2662
Publisher Cambridge Univ Press (Cambridge)
Affiliations UCL - SC/CHIM - Département de chimie
UCL - AGRO/BAPA - Département de biologie appliquée et des productions agricoles
Keywords Intestinal Inflammation ; Nuclear Factor-kappa B ; Inhibitor Of Kappa B Pathway ; Interleukin-8 Production ; Polyphenols ; Caco-2 Cells
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Bibliographic reference Romier, Beatrice ; Van De Walle, Jacqueline ; During, Alexandrine ; Larondelle, Yvan ; Schneider, Yves-Jacques. Modulation of signalling nuclear factor-kappa B activation pathway by polyphenols in human intestinal Caco-2 cells.23rd International Conference on Polyphenols (Winnipeg(Canada), Aug, 2006). In: The British Journal of Nutrition : an international journal of nutritional science, Vol. 100, no. 3, p. 542-551 (2008)
Permanent URL http://hdl.handle.net/2078.1/59152