Vandenberghe, Y.
Tee, L.
Morel, F.
Rogiers, V.
Guillouzo, A.
Yeoh, G.
Previous studies, by using Northern blotting analyses, showed that phenobarbital (PB) affects the steady-state mRNA levels of glutathione S-transferase (GST) subunits 1/2, 3/4 and 7 in both conventional cultures of adult rat hepatocytes and co-cultures, with rat liver epithelial cells [Vandenberghe et al., 1989, FEBS Lett. 251, 59-64; Morel et al., 1989, FEBS Lett. 258, 99-102]. To determine whether PB acts at the transcriptional level, nuclear 'run-on' experiments using cDNA probes hybridizing to GST subunits 1/2, 3/4 and 7 mRNA were performed on purified nuclei isolated from control and PB treated hepatocytes seeded under conventional and co-culture conditions. Data from this study demonstrate that the increase in steady-state mRNA levels observed in both conventional culture and co-culture after 4 days PB exposure results from an increased transcriptional activity of the GST genes. However, a substantial increase in steady-state mRNA levels in the absence of a commensurate increase in transcriptional activity at 12 days of co-culture, indicates that the barbiturate has also a stabilizing effect in vitro on the GST mRNAs.
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Bibliographic reference |
Vandenberghe, Y. ; Tee, L. ; Morel, F. ; Rogiers, V. ; Guillouzo, A. ; et. al. Regulation of Glutathione-s-transferase Gene-expression By Phenobarbital in Cultured Adult-rat Hepatocytes. In: FEBS Letters, Vol. 284, no. 1, p. 103-108 (1991) |
Permanent URL |
http://hdl.handle.net/2078.1/51035 |