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Murine dendritic cells pulsed in vitro with tumor antigen induce tumor resistance in vivo

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Flamand, Véronique Sornasse, Thierry Thielemans, Kris Demanet, Christian Bakkus, Marleen Bazin, Hervé [UCL] Moser, Muriel

The aim of this work is to induce tumor resistance to a B cell lymphoma in BALB/c mice using elements of the immune system. It has indeed been shown by us and by others that antigen-presenting cells (APC) like dendritic cells can induce efficient immune responses and can even substitute for Freund's adjuvant. Here we show that mice immunized with syngeneic dendritic cells pulsed in vitro with tumor antigen (BCL1 idiotype expressed by lymphoma cells) are protected against a subsequent tumor inoculation. The in vivo resistance can be correlated with the induction of a humoral response specific for the idiotype expressed by the tumor. No such protection can be achieved when B cells are used as APC. These data show that effector cells in tumor-bearing animals can be recruited and activated using dendritic cells, providing long-lasting immune surveillance.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Language Anglais
Journal information "European Journal of Immunology" - Vol. 24, no. 3, p. 605-610 (1994)
Peer reviewed yes
Publisher Wiley - V C H Verlag GmbH & Co. KGaA (Weinheim)
issn 0014-2980
e-issn 1521-4141
Publication status Publié
Affiliation UCL - MD/CHIR - Département de chirurgie
Keywords B cell lymphoma ; Antigen presentation ; Dendritic cells ; Immunotherapy
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Bibliographic reference Flamand, Véronique ; Sornasse, Thierry ; Thielemans, Kris ; Demanet, Christian ; Bakkus, Marleen ; et. al. Murine dendritic cells pulsed in vitro with tumor antigen induce tumor resistance in vivo. In: European Journal of Immunology, Vol. 24, no. 3, p. 605-610 (1994)
Permanent URL http://hdl.handle.net/2078.1/49052