Vanhulle, Sophie
[UCL]
The use of white rot fungi (WRF) is a promising alternative to treat dyes containing wastewaters. Six WRF strains were selected with regards to their ability of decolourisation and detoxification of azoic and anthraquinonic dyes: Coriolopsis polyzona (CP36), Perenniporia ochroleuca (PO33), Pycnoporus sanguineus (PS7), Perenniporia tephropora (PT32), and Trametes versicolor (TV17).
In presence of Acid Blue 62 (ABu62), depending on the strain, a cytoxicity reduction between 25 % and 85 % was observed on human cells. Laccase was shown as being effective in the cytotoxicity reduction. The major metabolites were isolated, characterized and a decolourisation mechanism was proposed. A dimerization of the initial molecule was followed by a degradation of this main molecule into smaller fragments. Depending of the dye, Cellobiose dehydrogenase displayed in vitro , a synergism or an antagonism with laccases in the decolourisation. The kinetic behaviour of an isoenzyme from PS7 was studied using ABTS and ABu62 as substrates. An activating effect was evidenced while at higher concentrations of dye, an inhibition was shown.
Bioremediation of an industrial effluent was studied and compared with ozonation. The effluent cytoxicity was reduced through ozonation and WRF respectively by 10% and 35% , whereas up to 70% cytotoxicity reduction was achieved by using a combined treatment. Mutagenicity, initially important in the raw effluent, could not be removed through ozonation, while the genotoxic effect was completely removed after fungal treatment.
Bibliographic reference |
Vanhulle, Sophie. White rot fungi in bioremediation of dyes. Prom. : Corbisier, Anne-Marie |
Permanent URL |
http://hdl.handle.net/2078.1/4587 |