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A qualitative method for assessing the C-14 labelling position in [C-14]-dimethyl aminoantipyrine (aminopyrine)

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Levêque, Philippe [UCL] Gallez, Bernard [UCL]

[C-14]-Aminopyrine is a well known compound which is widely used for the evaluation of the hepatic function (breath test). As this breath lest is based upon N-demethylation, it is essential to verify the labelling position in order to guarantee relevant results. We propose an enzymatic method based on collection of (CO2)-C-14 produced after specific in vitro N-demethylation in a medium containing horseradish peroxidase, formaldehyde dehydrogenase and formate dehydrogenase. This assay allows for a qualitative monitoring of the labelling position.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 1998
Language Anglais
Journal information "Journal of Labelled Compounds and Radiopharmaceuticals" - Vol. 41, no. 10, p. 959-963 (1998)
Peer reviewed yes
Publisher John Wiley & Sons Ltd (W Sussex)
issn 0362-4803
e-issn 1099-1344
Publication status Publié
Affiliations UCL - MD/MINT - Département de médecine interne
UCL - MD/FARM - Ecole de pharmacie
Keywords breath test ; aminopyrine ; qualify control
Links
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Bibliographic reference Levêque, Philippe ; Gallez, Bernard. A qualitative method for assessing the C-14 labelling position in [C-14]-dimethyl aminoantipyrine (aminopyrine). In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 41, no. 10, p. 959-963 (1998)
Permanent URL http://hdl.handle.net/2078.1/45010