User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Effect of repeated ethanol withdrawal on glutamate microdialysate in the hippocampus

Primary tabs

Dahchour, A. De Witte, Philippe [UCL]

Background: Our previous studies, which identified that ethanol withdrawal is associated with increases in glutamate microdialysate in the nucleus accumbens and reaches a maximum at 12 hr, have now been extended in order to assess whether repeated cycles of chronic ethanol intoxication followed by 12 hr withdrawal periods on three occasions alters glutamate release in the hippocampus of male rats. Methods: In this study, the microdialysis technique has been used with the HPLC and electrochemical detection. Results: During the first cycle of ethanol withdrawal, glutamate content increased significantly 8 hr after withdrawal (198.4% +/- 89.14%) by comparison with control rats. During the second period of ethanol withdrawal, 1 week after the initial withdrawal episode, glutamate microdialysate content increased significantly 10 hr after withdrawal, but to a much lower degree than in the first episode (179.08 +/- 25.68%), by comparison with control rats. During the third cycle of ethanol withdrawal, the concentration of glutamate in the hippocampus microdialysate did not significantly change at either of these time points except at 12 hr when glutamate was significantly decreased by comparison with control rats (52.09 +/- 14.38%). Apart from arginine, which was significantly decreased both at the cessation of alcoholization and during the 12 hr of the three withdrawal episodes, none of the other neurotransmitters assayed, aspartate, taurine, alanine, or GABA, showed any significant alteration. Conclusion: These results clearly indicate that elevated glutamate release during the first withdrawal episode is not paralleled in subsequent withdrawal episodes.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 1999
Language Anglais
Journal information "Alcoholism: Clinical and Experimental Research" - Vol. 23, no. 10, p. 1698-1703 (1999)
Peer reviewed yes
Publisher Lippincott Williams & Wilkins (Philadelphia)
issn 0145-6008
e-issn 1530-0277
Publication status Publié
Affiliation UCL
Keywords repeated ethanol withdrawal ; hippocampus ; glutamate ; microdialysis
Links
  1. Allan Andrea M., Harris R. Adron, Involvement of Neuronal Chloride Channels in Ethanol Intoxication, Tolerance, and Dependence, Recent Developments in Alcoholism (1987) ISBN:9781489916860 p.313-325, 10.1007/978-1-4899-1684-6_12
  2. Ballenger JC, Br J Psychiatry, 133, 1 (1987)
  3. Becker Howard C., Diaz-Granados Jaime L., Weathersby R.T., Repeated ethanol withdrawal experience increases the severity and duration of subsequent withdrawal seizures in mice, 10.1016/s0741-8329(97)87949-9
  4. Becker Howard C., Hale Robert L., Repeated Episodes of Ethanol Withdrawal Potentiate the Severity of Subsequent Withdrawal Seizures: An Animal Model of Alcohol Withdrawal "Kindling", 10.1111/j.1530-0277.1993.tb00731.x
  5. Bredt DS, Proc Natl Acad Sci USA, 87, 682 (1990)
  6. Chandler LG, J Neurochem, 60, 1578 (1993)
  7. Charness Michael E., Brain Lesions in Alcoholics, 10.1111/j.1530-0277.1993.tb00718.x
  8. Charness ME, N Engl J Med, 321, 442 (1989)
  9. CHOI D, Glutamate neurotoxicity and diseases of the nervous system, 10.1016/0896-6273(88)90162-6
  10. Choi Mikyung Kim, Fahrbach S. E., Demonstration of motoneuron-12 sparing in culturedManduca sexta ventral nerve cords, 10.1002/neu.480230404
  11. Dahchour A, Alcohol Clin Exp Res, 16, 6A (1996)
  12. Dahchour Abdelkader, De Witte Philippe, Bolo Nicolas, Nédélec Jean-François, Muzet Muriel, Durbin Philippe, Macher Jean-Paul, Central effects of acamprosate: Part 1. Acamprosate blocks the glutamate increase in the nucleus accumbens microdialysate in ethanol withdrawn rats, 10.1016/s0925-4927(98)00016-x
  13. Davidson Mark D., Wilce Peter, Shanley Brian C., Increased sensitivity of the hippocampus in ethanol-dependent rats to toxic effect ofN-methyl-D-aspartic acid in vivo, 10.1016/0006-8993(93)91562-7
  14. Dolin S, J Pharmacol Exp Ther, 250, 985 (1989)
  15. Dolin S., Little H., Hudspith M., Pagonis C., Littleton J., Increased dihydropyridine-sensitive calcium channels in rat brain may underlie ethanol physical dependence, 10.1016/0028-3908(87)90220-6
  16. Donzanti Bruce A., Yamamoto Bryan K., An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates, 10.1016/0024-3205(88)90267-6
  17. Douglas RM, Psychol Bull, 67, 416 (1967)
  18. Grant Kathleen A., Valverius Peter, Hudspith Michael, Tabakoff Boris, Ethanol withdrawal seizures and the NMDA receptor complex, 10.1016/0014-2999(90)90022-x
  19. Greenberg DA, Brain Res, 410, 143 (1987)
  20. Gulya Karoly, Grant Kathleen A., Valverius Peter, Hoffman Paula L., Tabakoff Boris, Brain regional specificity and time-course of changes in the NMDA receptor-ionophore complex during ethanol withdrawal, 10.1016/0006-8993(91)90583-h
  21. Hoffman Paula L., lorio Karen R., Snell Lawrence D., Tabakoff Boris, Attenuation of Glutamate-Induced Neurotoxicity in Chronically Ethanol-Exposed Cerebellar Granule Cells by NMDA Receptor Antagonists and Ganglioside GM1, 10.1111/j.1530-0277.1995.tb01573.x
  22. PL Hoffman, SC Rabe, P Valverius, KA Grant, K Gulya, JC Crabbe, and B Tabakoff (1990 ) The NMDA receptor is implicated in the acute effect of ethanol and ethanol withdrawal. ISBRA, Toronto.
  23. Hunt Walter A., Are binge drinkers more at risk of developing brain damage?, 10.1016/0741-8329(93)90083-z
  24. Iorio KR, Eur J Pharmacol, 248, 209 (1993)
  25. Lallemand F, Alcohol Clin Exp Res, 22, 189A (1998)
  26. Bourhis B, C R Seances Soc Biol, 169, 898 (1975)
  27. Lynch Marina A., Littleton John M., Possible association of alcohol tolerance with increased synaptic Ca2+ sensitivity, 10.1038/303175a0
  28. Marks SS, J Neurochem, 53, 168 (1989)
  29. McCown Thomas J., Breese George R., Multiple Withdrawals from Chronic Ethanol "Kindles" Inferior Collicular Seizure Activity: Evidence for Kindling of Seizures Associated with Alcoholism, 10.1111/j.1530-0277.1990.tb00492.x
  30. Messing RO, Proc Natl Acad Sci USA, 83, 6213 (1986)
  31. Paxinos G, The Rat Brain, in Stereotaxic Coordinates (1982)
  32. Peterson Christine, Neal John H., Cotman Carl W., Development of excitotoxicity in cultured hippocampal neurons, 10.1016/0165-3806(89)90075-8
  33. Poldrugo Flavio, Snead O. Carter, Electroencephalographic and behavioral correlates in rats during repeated ethanol withdrawal syndromes, 10.1007/bf00429722
  34. Rastogi S, Effect of chronic treatment of ethanol on benzodiazepine and picrotoxin sites on the GABA receptor complex in regions of the brain of the rat, 10.1016/0028-3908(86)90167-x
  35. Regan R.F., Choi D.W., Glutamate neurotoxicity in spinal cord cell culture, 10.1016/0306-4522(91)90317-h
  36. Robinson Terry E., Whishaw Ian Q., Normalization of extracellular dopamine in striatum following recovery from a partial unilateral 6-OHDA lesion of the substantia nigra: a microdialysis study in freely moving rats, 10.1016/0006-8993(88)91560-0
  37. Rossetti Zvani L., Carboni Susanna, Ethanol withdrawal is associated with increased extracellular glutamate in the rat striatum, 10.1016/0014-2999(95)00344-k
  38. Rottenberg Hagai, Alcohol modulation of benzodiazepine receptors, 10.1016/0741-8329(85)90046-1
  39. Ruhe CAM, Alcohol Alcohol Suppl, 2, 217 (1994)
  40. Skattebøl Atle, Rabin Richard A., Effects of ethanol on 45Ca2+ uptake in synaptosomes and in PC12 cells, 10.1016/0006-2952(87)90156-0
  41. Tavares M. A., Paula-Barbosa M. M., Cadete-Leite A., Chronic Alcohol Consumption Reduces the Cortical Layer Volumes and the Number of Neurons of the Rat Cerebellar Cortex, 10.1111/j.1530-0277.1987.tb01315.x
  42. Traub Roger D., Miles Richard, Neuronal networks of the hippocampus, ISBN:9780511895401, 10.1017/cbo9780511895401
  43. Veatch Lynn M., Gonzalez Larry P., Repeated Ethanol Withdrawal Produces Site-Dependent Increases in EEG Spiking, 10.1111/j.1530-0277.1996.tb01638.x
  44. Walker DW, Science, 209, 711 (1980)
  45. Whittington M.A., Dolin S.J., Patch T.L., Siarey R.J., Butterworth A.R., Little H.J., Chronic dihydropyridine treatment can reverse the behavioural consequences of and prevent adaptations to, chronic ethanol treatment, 10.1111/j.1476-5381.1991.tb09845.x
  46. Zhang Ying, Crichton Robert R., Boelaert Johan R., Jorens Philippe G., Herman Arnold G., Ward Roberta J., Lallemand Frédéric, de Witte Philippe, Decreased release of nitric oxide (NO) by alveolar macrophages after in vivo loading of rats with either iron or ethanol, 10.1016/s0006-2952(97)00382-1
Bibliographic reference Dahchour, A. ; De Witte, Philippe. Effect of repeated ethanol withdrawal on glutamate microdialysate in the hippocampus. In: Alcoholism: Clinical and Experimental Research, Vol. 23, no. 10, p. 1698-1703 (1999)
Permanent URL http://hdl.handle.net/2078.1/44113