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Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks

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Hayes, F Hallet, Bernard [UCL]

Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.

Document type Article de périodique (Journal article) – Synthèse de littérature
Access type Accès restreint
Publication date 2000
Language Anglais
Journal information "Trends in Microbiology" - Vol. 8, no. 12, p. 571-577 (2000)
Peer reviewed yes
Publisher Elsevier Science London (London)
issn 0966-842X
e-issn 1878-4380
Publication status Publié
Affiliation UCL - SC/BIOL - Département de biologie
Links
Bibliographic reference Hayes, F ; Hallet, Bernard. Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks. In: Trends in Microbiology, Vol. 8, no. 12, p. 571-577 (2000)
Permanent URL http://hdl.handle.net/2078.1/42970