Hayes, F
Hallet, Bernard
[UCL]
Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.
Bibliographic reference |
Hayes, F ; Hallet, Bernard. Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks. In: Trends in Microbiology, Vol. 8, no. 12, p. 571-577 (2000) |
Permanent URL |
http://hdl.handle.net/2078.1/42970 |