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Influence of the growth substrate on the mycolic acid profiles of mycobacteria

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Wick, LY Wattiau, Pierre [UCL] Harms, H

The influence of growth substrates on mycolic acid profiles of PAH-degrading Mycobacterium spp. LB501T, LB307T and VM552 was examined by high-performance liquid chromatography (HPLC) using glucose, alkanes, polycyclic aromatic hydrocarbons (PAH) or Luria-Bertani medium (LB) as the sole carbon source. The substrates gave rise to varying mycolic acid profiles, as bacteria growing on poorly water-soluble substrates exhibited more hydrophobic mycolic acids than cells grown on glucose. Our results indicate that mycobacteria respond to the growth substrate by changing the mycolic acid composition of their cell wall, pointing at the importance of the growth substrate for mycolic acid profiling as an identification method of actinomycetes.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2002
Language Anglais
Journal information "Environmental Microbiology" - Vol. 4, no. 10, p. 612-616 (2002)
Peer reviewed yes
Publisher Blackwell Publishing Ltd (Oxford)
issn 1462-2912
e-issn 1462-2920
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Links
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Bibliographic reference Wick, LY ; Wattiau, Pierre ; Harms, H. Influence of the growth substrate on the mycolic acid profiles of mycobacteria. In: Environmental Microbiology, Vol. 4, no. 10, p. 612-616 (2002)
Permanent URL http://hdl.handle.net/2078.1/41651