Celecoxib has a positive effect on the overall metabolism of hyaluronan human osteoarthritic and proteoglycans in cartilage

Objective. To assess the effects of celecoxib, a cyclooxygenase (COX-2) selective inhibitor, on the metabolism of hyaluronan (HA) and proteoglycans (PG) in human cartilage explants with midrange seventy of osteoarthritis (OA). Results were compared with those of diclofenac, a non-selective COX inhibitor. Methods. Cartilage specimens (OA grade 4-8 on Mankin's scale) were pulsed with H-3-glucosamine and chased in the absence or presence of 1-10 mug/ml of celecoxib or diclofenac. After papain digestion, the labeled chondroitin sulfate and HA molecules were purified by anion-exchange chromatography. Results. Diclofenac did not affect the metabolic balance of PG and HA whereas, in a relatively dose-dependent manner, celecoxib increased the synthesis of HA and PG; celecoxib also reduced the net loss of labeled HA and PG molecules from cartilage explants. Conclusion. In short term in vitro cultures, celecoxib has a favorable effect on the overall metabolism of PG and HA. It is therefore unlikely that this drug would have a detrimental effect on articular cartilage during longterm administration. Further, celecoxib might help counteract the depletion of HA seen in OA cartilage.