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Role of the interleukin (IL)-28 receptor tyrosine residues for antiviral and antiproliferative activity of IL-29/interferon-lambda 1 - Similarities with type I interferon signaling

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Dumoutier, Laure [UCL] Tounsi, Amel [UCL] Michiels, Thomas [UCL] Sommereyns, Caroline [UCL] Kotenko, SV Renauld, Jean-Christophe [UCL]

Interferon (IFN)-lambda1, -lambda2, and -lambda3 are the latest members of the class II cytokine family and were shown to have antiviral activity. Their receptor is composed of two chains, interleukin-28R/likely interleukin or cytokine or receptor 2 (IL-28R/LICR2) and IL-10Rbeta, and mediates the tyrosine phosphorylation of STAT1, STAT2, STAT3, and STAT5. Here, we show that activation of this receptor by IFN-lambda1 can also inhibit cell proliferation and induce STAT4 phosphorylation, further extending functional similarities with type I IFNs. We used IL-28R/LICR2-mutated receptors to identify the tyrosines required for STAT activation, as well as antiproliferative and antiviral activities. We found that IFN-lambda1-induced STAT2 tyrosine phosphorylation is mediated through tyrosines 343 and 517 of the receptor, which showed some similarities with tyrosines from type I IFN receptors involved in STAT2 activation. These two tyrosines were also responsible for antiviral and antiproliferative activities of IFN-lambda1. By contrast, STAT4 phosphorylation ( and to some extent STAT3 activation) was independent from IL-28R/LICR2 tyrosine residues. Taken together, these observations extend the functional similarities between IFN-lambdas and type I IFNs and shed some new light on the mechanisms of activation of STAT2 and STAT4 by these cytokines.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2004
Language Anglais
Journal information "Journal of Biological Chemistry" - Vol. 279, no. 31, p. 32269-74 (2004)
Peer reviewed yes
Publisher Amer Soc Biochemistry Molecular Biology Inc (Bethesda)
issn 0021-9258
e-issn 1083-351X
Publication status Publié
Affiliations UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
UCL - MD/MINT - Département de médecine interne
MESH Subject Up-Regulation ; Tyrosine - chemistry ; Transfection ; Trans-Activators - metabolism ; Signal Transduction ; STAT5 Transcription Factor ; STAT4 Transcription Factor ; STAT3 Transcription Factor ; STAT2 Transcription Factor ; STAT1 Transcription Factor ; Receptors, Cytokine - chemistry - metabolism ; Phosphorylation ; Milk Proteins ; Lymphoma, T-Cell - metabolism ; Luciferases - metabolism ; Interleukins - metabolism ; Interferon-gamma - metabolism ; Humans ; Dose-Response Relationship, Drug ; DNA-Binding Proteins - metabolism ; Cytokines - metabolism ; Cell Line, Tumor ; Cell Line ; Cell Division ; Blotting, Western ; Antiviral Agents - pharmacology
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Bibliographic reference Dumoutier, Laure ; Tounsi, Amel ; Michiels, Thomas ; Sommereyns, Caroline ; Kotenko, SV ; et. al. Role of the interleukin (IL)-28 receptor tyrosine residues for antiviral and antiproliferative activity of IL-29/interferon-lambda 1 - Similarities with type I interferon signaling. In: Journal of Biological Chemistry, Vol. 279, no. 31, p. 32269-74 (2004)
Permanent URL http://hdl.handle.net/2078.1/40055