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The clonal structure of Bacillus thuringiensis isolates from north-east Poland does not correlate with their cry gene diversity

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Swiecicka, I Mahillon, Jacques [UCL]

The genetic relationship among 103 natural Bacillus thuringiensis isolates was investigated on the basis of polymerase chain reaction amplification of their specific crystal (cry) protein type genes and chromosomal DNA profiling by pulsed-field gel electrophoresis (PFGE). The strains were recovered from the intestines of small wild rodents and insectivores from the Biebrza National Park and the Lomza Landscape Park of the Narew River Valley in north-east Poland. The percentage of B. thuringiensis strains harbouring genes coding for toxins active against Lepidoptera (cry1, cry2, cry9) was very high (64%) compared with that of Diptera-specific strains (cry4, 14%). No strain with cry genes coding for proteins directed against coleopteran larvae and nematodes was found. After digestion with NotI and AscI, only nine PFGE pulsotypes were observed among all isolates, indicating a clonal structure for the B. thuringiensis population from NE Poland. Interestingly, no correlation was observed between the DNA pulsotype strains and their crystal gene content and diversity. These results therefore emphasize the importance of cry gene horizontal transfer occurring among natural isolates of B. thuringiensis.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2005
Language Anglais
Journal information "Environmental Microbiology" - Vol. 7, no. 1, p. 34-39 (2005)
Peer reviewed yes
Publisher Blackwell Publishing Ltd (Oxford)
issn 1462-2912
e-issn 1462-2920
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Links
  1. Bravo A., J Bacteriol, 179, 2793 (1997)
  2. Schnepf E., Microbiol Mol Biol Rev, 62, 775 (1998)
  3. N. Crickmore (2004 )Bacillus thuringiensistoxin nomenclature. [WWW document] URL http://www.biols.susx.ac.uk/home/Neil_Crickmore/Bt/
  4. Ferre J., Proc Natl Acad Sci USA, 88, 5119 (1991)
  5. Herron Grant A, Powis Kevin, Rophail Jeanette, Insecticide resistance inAphis gossypiiGlover (Hemiptera: Aphididae), a serious threat to Australian cotton, 10.1046/j.1440-6055.2001.00200.x
  6. Tabashnik B. E., Liu Y.-B., de Maagd R. A., Dennehy T. J., Cross-Resistance of Pink Bollworm (Pectinophora gossypiella) to Bacillus thuringiensis Toxins, 10.1128/aem.66.10.4582-4584.2000
  7. Jouanin Lise, Bonadé-Bottino Michel, Girard Cécile, Morrot Gil, Giband Marc, Transgenic plants for insect resistance, 10.1016/s0168-9452(97)00239-2
  8. Leroy T., Henry A. -M., Royer M., Altosaar I., Frutos R., Duris D., Philippe R., Genetically modified coffee plants expressing the Bacillus thuringiensis cry 1Ac gene for resistance to leaf miner, 10.1007/s002990050744
  9. Groot Astrid T., Dicke Marcel, Insect-resistant transgenic plants in a multi-trophic context, 10.1046/j.1365-313x.2002.01366.x
  10. Ben-Dov E., Appl Environ Microbiol, 63, 4883 (1997)
  11. Juarez-Perez V.M., Appl Environ Microbiol, 63, 2997 (1997)
  12. Bravo A., Appl Environ Microbiol, 64, 4965 (1998)
  13. Carlson C, The chromosome map of Bacillus thuringiensis subsp. canadensis HD224 is highly similar to that of the Bacillus cereus type strain ATCC 14579, 10.1016/0378-1097(96)00216-9
  14. Gaviria Rivera Adelaida M, Priest Fergus G, Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure toBacillus thuringiensisthat relates in general to crystal protein gene content, 10.1016/s0378-1097(03)00347-1
  15. Swiecicka I., Fiedoruk K., Bednarz G., The occurrence and properties of Bacillus thuringiensis isolated from free-living animals, 10.1046/j.1472-765x.2002.01070.x
  16. ?wicicka Izabela, Molecular Typing by Pulsed-Field Gel Electrophoresis of Bacillus thuringiensis from Root Voles, 10.1007/s00284-002-3871-x
  17. Martinez C., Caballero P., Contents of cry genes and insecticidal toxicity of Bacillus thuringiensis strains from terrestrial and aquatic habitats, 10.1046/j.1365-2672.2002.01579.x
  18. Damgaard Per H., Natural occurrence and dispersal of Bacillus thuringiensis in the environment, Entomopathogenic Bacteria: from Laboratory to Field Application (2000) ISBN:9789048155422 p.23-40, 10.1007/978-94-017-1429-7_2
  19. Chak K.F., Appl Environ Microbiol, 60, 2415 (1994)
  20. Ben-Dov E., Appl Environ Microbiol, 65, 3714 (1999)
  21. Helgason E., J Clin Microbiol, 38, 1615 (2000)
  22. Ankarloo Jonas, Caugant Dominique A., Hansen Bjarne M., Berg Alexandra, Kolstø Anne-Brit, Lövgren Ann, Genome Stability of Bacillus thuringiensis subsp. israelensis Isolates, 10.1007/s002849910010
  23. Zahner Viviane, Momen Hoomen, Priest Fergus G., Serotype H5a5b is a major clone within mosquito-pathogenic strains of Bacillus sphaericus, 10.1016/s0723-2020(98)80020-9
  24. Macdonald R., Appl Environ Microbiol, 61, 2446 (1995)
  25. Kolsto Anne-Brit, Dynamic bacterial genome organization, 10.1046/j.1365-2958.1997.3501715.x
  26. Rosso M.-L., Entomopathogenic Bacteria: from Laboratory to Field Application, 267 (2000)
  27. Helgason *, † , Dominique A. C Erlendur, Lecadet Marguerite-M., Chen Yahua, Mahillon Jacques, Lövgren Ann, Hegna Ida, Kvaløy Kirsti, Kolstø Anne-Brit, Genetic Diversity of Bacillus cereus / B. thuringiensis Isolates from Natural Sources, 10.1007/s002849900343
  28. Vilas-Boas G., Sanchis V., Lereclus D., Lemos M. V. F., Bourguet D., Genetic Differentiation between Sympatric Populations of Bacillus cereus and Bacillus thuringiensis, 10.1128/aem.68.3.1414-1424.2002
  29. Jarrett P., Appl Environ Microbiol, 56, 1608 (1990)
  30. Thomas D.J.I., Appl Environ Microbiol, 66, 118 (2000)
  31. Hu Xiaomin, Hansen Bjarne Munk, Eilenberg J�rgen, Hendriksen Niels Bohse, Smidt Lasse, Yuan Zhiming, Jensen Gert Bolander, Conjugative transfer, stability and expression of a plasmid encoding acry1Ac gene inBacillus cereusgroup strains, 10.1016/s0378-1097(03)00925-x
  32. Bickley J., Diagnostic Bacteriology Protocols, 46, 141 (1995)
Bibliographic reference Swiecicka, I ; Mahillon, Jacques. The clonal structure of Bacillus thuringiensis isolates from north-east Poland does not correlate with their cry gene diversity. In: Environmental Microbiology, Vol. 7, no. 1, p. 34-39 (2005)
Permanent URL http://hdl.handle.net/2078.1/39654