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Cancer-germline gene expression in pediatric solid tumors using quantitative real-time PCR

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Jacobs, Joannes F. M. Brasseur, Francis [UCL] Hulsbergen-van de Kaa, Christina A. van de Rakt, Mandy W. M. M. Figdor, Carl G. Coulie, Pierre G. [UCL] de Vries, I. Jolanda M.

Cancer-germline genes (CGGs) code for immunogenic antigens that are present on various human tumors but not on normal tissues. The importance of CGGs in cancer immunotherapy has led to detailed studies of their expression in a range of human tumors. We measured the levels of expression of 12 CGGs in various pediatric solid tumors to identify targets for therapeutic cancer vaccines. Quantitative real-time PCR (qPCR) was used to measure the expression of 8 MAGE genes and of genes LAGE-2/NY-ESO-1 and GAGE-1, 2, 8 in 9 osteosarcomas, 10 neuroblastomas, 12 rhabdomyosarcomas and 18 Ewing's sarcomas. Nine tumors were also examined by immunohistochemistry with monoclonal antibodies specific for the MAGE-A1, MAGE-A4 and NY-ESO-1 proteins. All osteosarcoma and 80% of neuroblastoma samples expressed several CGGs at high levels. Six of 12 rhabdomyosarcomas and 11 of 18 Ewing's sarcomas expressed at least one CGG. Immunohistochemistry data correlated well with qPCR results and showed a homogeneous protein distribution pattern in most positive tumors. No correlation was found between the levels of CGG expression in the tumors and clinicopathological parameters of the patients. Pediatric solid tumors express several CGGs, which encode antigens that could be targeted in therapeutic vaccination trials. Several CGGs of the MAGE, GAGE and LAGE families are coexpressed in a large proportion of osteosarcoma and neuroblastoma samples. Some rhabdomyosarcomas express several of these genes at high levels. Ewing's sarcomas have an overall low CGG expression. (c) 2006 Wiley-Liss, Inc.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2007
Language Anglais
Journal information "International Journal of Cancer" - Vol. 120, no. 1, p. 67-74 (2007)
Peer reviewed yes
Publisher Wiley-liss (Hoboken)
issn 0020-7136
e-issn 1097-0215
Publication status Publié
Affiliations UCL - MD/BICL - Département de biochimie et de biologie cellulaire
UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Unité d'oncologie médicale
MESH Subject Testis - metabolism ; Sarcoma, Ewing's - genetics - metabolism ; Rhabdomyosarcoma - genetics - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Osteosarcoma - genetics - metabolism ; Neuroblastoma - genetics - metabolism ; Neoplasms - genetics - metabolism ; Neoplasm Proteins - genetics - metabolism ; Membrane Proteins - genetics - metabolism ; Male ; Infant, Newborn ; Infant ; Immunoenzyme Techniques ; Humans ; Gene Expression Regulation, Neoplastic ; Child, Preschool ; Child ; Bone Neoplasms - genetics - metabolism ; Antigens, Neoplasm - genetics - metabolism ; Adult ; Adolescent
Keywords cancer ; pediatrics ; qPCR ; MAGE ; NY-ESO-1
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Bibliographic reference Jacobs, Joannes F. M. ; Brasseur, Francis ; Hulsbergen-van de Kaa, Christina A. ; van de Rakt, Mandy W. M. M. ; Figdor, Carl G. ; et. al. Cancer-germline gene expression in pediatric solid tumors using quantitative real-time PCR. In: International Journal of Cancer, Vol. 120, no. 1, p. 67-74 (2007)
Permanent URL http://hdl.handle.net/2078.1/38090