To set up a rational collecting strategy for germplasm of the edible-seeded cucurbit Cucumeropsis mannii, a study was conducted using 24 morphological and seven putative enzyme markers to determine the intra-specific variability from 16 and 22 accessions (representing three cultivars), respectively. The analysis of variance, showed a significant difference between the three cultivars. Principal component analysis pointed out a variation among individuals, mainly on the basis of flower, fruit, and seed size. Dendrogram with UPGMA method allowed clustering of the cultivars. Genetic diversity indices estimated equalled: 9.96% for the proportion of polymorphic loci (P), 1.10 for the number of alleles (A) and 0.023 for observed heterozygosity (H-o). The level of the within accessions genetic diversity (H-S=0.078) was higher than among accessions (D-ST=0.042). Nei's genetic distances between the three cultivars were also low (0.079-0.147), indicating a high degree of similarity of the analysed cultivars. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.
Koffi, Kouame K. ; Gbotto, Ahou A. ; Malice, Marie ; Dje, Yao ; Bertin, Pierre ; et. al. Morphological and allozyme variation in a collection of Cucumeropsis mannii Naudin (Cucurbitaceae) from Cote d'Ivoire. In: Biochemical Systematics and Ecology, Vol. 36, no. 10, p. 777-789 (2008)