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Surface composition of insulin and albumin adsorbed on polymer substrates as revealed by multivariate analysis of ToF-SIMS data

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Henry, Marie [UCL] Bertrand, Patrick [UCL]

The structure and the composition of adsorbed protein films containing human serum albumin (HSA) and/or human insulin were studied with time-of-flight secondary ion mass spectrometry (ToF-SIMS). The proteins were adsorbed either on native polycarbonate (PC) membranes or on PC membranes treated with poly(N-vinylpyrrolidone) (PVP/PC). These membranes have been developed for their use as encapsulation membranes in a bioartificial pancreas. Principal component analysis (PCA) of the ToF-SIMS results allowed the determination of the main factors affecting the surface structure and composition of the protein layer. The substrate surface properties were found to be the most influencing factor. The amount and type of the adsorbed protein also influence the adsorbed protein layer surface composition and structure. A partial least squares (PLS) regression model allowed confirmation of the correlation between the adsorbed amount of protein and the surface composition of the protein layer. Copyright (C) 2008 John Wiley & Sons, Ltd.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2009
Language Anglais
Journal information "Surface and Interface Analysis" - Vol. 41, no. 2, p. 105-113 (2009)
Peer reviewed yes
Publisher John Wiley & Sons Ltd (Chichester)
issn 0142-2421
e-issn 1096-9918
Publication status Publié
Affiliation UCL
Keywords ToF-SIMS ; protein adsorption ; principal component analysis ; partial least squares regression ; polycarbonate membrane
Links
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Bibliographic reference Henry, Marie ; Bertrand, Patrick. Surface composition of insulin and albumin adsorbed on polymer substrates as revealed by multivariate analysis of ToF-SIMS data. In: Surface and Interface Analysis, Vol. 41, no. 2, p. 105-113 (2009)
Permanent URL http://hdl.handle.net/2078.1/35840