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Surface Chemical Composition of Diatoms

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Tesson, Benoit Genet, Michel [UCL] Fernandez, Vincent Degand, Simon [UCL] Rouxhet, Paul [UCL] Martin-Jezequel, Veronique

Among diatoms, Phaeodactylum tricornutum is a peculiar species that exists in three morphotypes with distinct cell wall structures and low silica context. X-ray photoelectron spectroscopy (XPS) analysis was performed on P. tricornutum and compared with diatom Thalassiosira pseudonana, the result provide new information on the chemical composition (elements, chemical functions, classes of biochemical compounds) of the cell surface. Two types of silicon were found condensed-silica (SiO2) and weakly polymerised silicate. Cells of T. pseudonana showed the highest concentration of silicon, with a majority in the form of condensed silica. For the fusiform and triradiate morpotypes of P. tricornutum, the majority of the small concentration of silican found was in the form of weakly polymerised silica. for all morphotypes of P. tricornutum, higher polysaccharide concentrations replaced silicas as a structural part of the cell wall. In both diatoms a high concentration of lipids was measured, in the form of carboxylic esters. Protonated nitrogen and phosphate were found in correlated amounts and attributed not only to phospholipids but also to phosphoproteins. Chloride ions characterised by a high electron density might be associated to the moieties. Sulfate groups were also detected, principally in P. tricornutum, and attributed to monoesters of polysaccharides.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2009
Language Anglais
Journal information "ChemBioChem : a European journal of chemical biology" - Vol. 10, no. 12, p. 2011-2024 (2009)
Peer reviewed yes
Publisher Wiley-v C H Verlag Gmbh (Weinheim)
issn 1439-4227
e-issn 1439-7633
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Keywords biomineralization ; diatoms ; silica ; silicates ; X-ray photoelectron spectroscopy
Links
  1. Nelson, Global Biogeochem. Cycles, 9, 359 (1995)
  2. Schmid, Protoplasma, 181, 43 (1994)
  3. Chiovitti, J. Phycol., 41, 1154 (2005)
  4. McConville, Protoplasma, 206, 188 (1999)
  5. Percival, Phytochemistry, 19, 809 (1980)
  6. Kröger, Eur. J. Biochem., 239, 259 (1996)
  7. Kates, Zeitschrift Pflanzenphysiol., 60, 19 (1968)
  8. Tesson, Anal. Bioanal. Chem., 390, 1889 (2008)
  9. Martin-Jezequel, J. Phycol., 36, 821 (2000)
  10. Martin-Jézéquel, Progress in Molecular and Subcellular Biology, Vol. 33: Silicon Biomineralization: Biology, Biochemistry, Biotechnology, 99 (2003)
  11. Swift, J. Phycol., 28, 202 (1992)
  12. Poulsen, J. Biol. Chem., 279, 42993 (2004)
  13. Sumper, Adv. Funct. Mater., 16, 17 (2006)
  14. Wenzl, Angew. Chem., 120, 1753 (2008)
  15. Angew. Chem. Int. Ed. 2008, 47, 1729.
  16. Borowitzka, J. Phycol., 14, 10 (1978)
  17. De Martino, J. Phycol., 43, 992 (2007)
  18. Tesson, Botanica Marina, 52, 104 (2009)
  19. Francius, Environ. Microbiol., 10, 1344 (2008)
  20. Lewin J. C., Lewin R. A., Philpott D. E., Observations on Phaeodactylum tricornutum, 10.1099/00221287-18-2-418
  21. Volcani B. E., Cell Wall Formation in Diatoms: Morphogenesis and Biochemistry, Silicon and Siliceous Structures in Biological Systems (1981) ISBN:9781461259466 p.157-200, 10.1007/978-1-4612-5944-2_7
  22. Reimann, J. Ultrastruct. Res., 21, 182 (1967)
  23. Dugdale, Biophys. J., 90, L58 (2006)
  24. Abdullahi, J. Phycol., 42, 363 (2006)
  25. Kröger, Curr. Opin. Chem. Biol., 11, 662 (2007)
  26. Sumper, J. Mater. Chem., 14, 2059 (2004)
  27. Hildebrand, Eur. J. Physiol., 456, 127 (2008)
  28. Hildebrand, J. Mater. Res., 21, 2689 (2006)
  29. Davis, Met. Ions Life Sci., 4, 255 (2008)
  30. Hildebrand, Chem. Rev., 108, 4855 (2008)
  31. Chiovitti, Eur. J. Phycol., 38, 351 (2003)
  32. Chiovitti, Eur. J. Phycol., 39, 117 (2004)
  33. Frigeri Luciano G., Radabaugh Timothy R., Haynes Paul A., Hildebrand Mark, Identification of Proteins from a Cell Wall Fraction of the DiatomThalassiosira pseudonana : Insights into Silica Structure Formation, 10.1074/mcp.m500174-mcp200
  34. Hecky, Mar. Biol., 19, 323 (1973)
  35. Rouxhet, Colloids Surf. B, 2, 347 (1994)
  36. Genet, Medical Applications of Colloids, 177 (2008)
  37. Dengis, Colloids Surf. B, 4, 199 (1995)
  38. van der Mei, Surf. Sci. Rep., 39, 1 (2000)
  39. Baddiley, Nature, 243, 43 (1973)
  40. Figueira, Environ. Sci. Technol., 33, 1840 (1999)
  41. Lin, Spectrochim. Acta, 61, 1195 (2005)
  42. Pethkar, Bioresour. Technol., 80, 211 (2001)
  43. Dufrêne, Colloids Surf. B, 7, 271 (1996)
  44. Dufrêne, Can. J. Microbiol., 42, 548 (1996)
  45. Tanuma, Surf. Interface Anal., 21, 165 (1994)
  46. http://www.quases.com, QUASES-IMFP-TPP2M Version 2.1,S. Tougaard.
  47. Paynter, Surface and Interfacial Aspects of Biomedical Polymers, Vol. 2: Protein Adsorption, 189 (1985)
  48. Isleib, Am. J. Potato Res., 35, 428 (1958)
  49. Ahimou, J. Colloid Interface Sci., 309, 49 (2007)
  50. NIST X-ray Photoelectron Spectroscopy Database, Version 4.0 (National Institute of Standards and Technology, Gaithersburg 2008); http://srdata.nist.gov./xps2
  51. Caillou Samuel, Boonaert Christophe J. P., Dewez Jean-Luc, Rouxhet Paul G., Oxidation of proteins adsorbed on hemodialysis membranes and model materials, 10.1002/jbm.b.30866
  52. Ishikawa, Langmuir, 7, 596 (1991)
  53. Black, Cement and Concrete Research, 36, 1023 (2006)
  54. Christiansen, Solid State Nucl. Magn. Reson., 29, 170 (2006)
  55. Gröger, J. Struct. Biol., 161, 55 (2008)
  56. Kinrade, J. Chem. Soc. Dalton Trans., 307 (2002)
  57. Gerin, J. Chem. Phys., 92, 1043 (1995)
  58. Boonaert, Appl. Environ. Microbiol., 66, 2548 (2000)
  59. Dengis, J. Microbiol. Methods, 26, 171 (1996)
  60. Ardizzone, Appl. Surf. Sci., 119, 253 (1997)
  61. Mekki, J. Non-Cryst. Solids, 220, 267 (1997)
  62. http://genome.jgi-psf.org/Phatr2/Phatr2.home.html
  63. Sumper, Angew. Chem., 119, 8557 (2007)
  64. Angew. Chem. Int. Ed. 2007, 46, 8405.
  65. Pickett-Heaps, The Cell Biology of Diatom Valve Formation, Vol. 7 (1990)
  66. Gélabert, Geochim. Cosmochim. Acta, 68, 4039 (2004)
  67. Rouxhet, Surf. Interface Anal., 40, 718 (2008)
  68. Iwasa, Proc. Int. Seaweed Symp., 7, 319 (1971)
  69. Gutenbrunner, J. Phycol., 30, 129 (1994)
  70. Stanley, Eur. J. Phycol., 42, 191 (2007)
  71. Smith, J. Phycol., 36, 321 (2000)
  72. Tesson, Mar. Chem., 109, 60 (2008)
  73. Ford, J. Chem. Soc., 1298, 7042 (1965)
  74. Percival, Chemistry and Enzymology of Marine Algal Polysaccharide (1967)
  75. Bertermann, Anal. Bioanal. Chem., 375, 630 (2003)
  76. Neuville, C. R. Acad. Sci. Paris, 273, 1221 (1971)
  77. Johansen, Great Basin Nat., 51, 310 (1991)
  78. Armbrust, Science, 306, 79 (2004)
  79. Guillard, Can. J. Microbiol., 8, 229 (1962)
  80. Strickland, A Practical Handbook of Seawater Analysis (1972)
  81. Dengis, Yeast, 13, 931 (1997)
Bibliographic reference Tesson, Benoit ; Genet, Michel ; Fernandez, Vincent ; Degand, Simon ; Rouxhet, Paul ; et. al. Surface Chemical Composition of Diatoms. In: ChemBioChem : a European journal of chemical biology, Vol. 10, no. 12, p. 2011-2024 (2009)
Permanent URL http://hdl.handle.net/2078.1/35357