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Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes

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Elisashvili, Vladimir [Durmishidze Inst Biochem & Biotechnol, GE-0159 Tbilisi, Rep of Georgia] Kachlishvili, Eva [Durmishidze Inst Biochem & Biotechnol, GE-0159 Tbilisi, Rep of Georgia] Khardziani, Tamar [Durmishidze Inst Biochem & Biotechnol, GE-0159 Tbilisi, Rep of Georgia] Agathos, Spiros N. [UCL]

Three white-rot fungi displayed a wide diversity in their response to supplemented aromatic compounds. Pyrogallol stimulated Cerrena unicolor laccase and manganese peroxidase (MnP) synthesis in synthetic medium 2.5- and 2-fold, respectively, whereas 2,4,6-trinitrotoluene (TNT) brought about a 2.8-fold increase in laccase yield by Trametes versicolor in submerged fermentation of ethanol production residue. No effect of the tested aromatic compounds on enzyme secretion by Ganoderma lucidum in mannitol-containing medium was detected. Nevertheless, G. lucidum is a potent producer of laccase in submerged fermentation of wheat bran and enzyme synthesis can be further increased by supplementation of medium with an appropriate inducer. The structure and the concentration of aromatic compounds play an important role in the regulation of enzyme synthesis. The supplementation of synthetic medium with 0.03-0.3 mM TNT or hydroquinone increased the differential rate of laccase synthesis by C. unicolor from 1,267 to 3,125-8,630 U mg biomass(-1) day(-1). Moreover, the same aromatic compound may function as either an inducer or a repressor, depending on the fungus and enzyme studied. Thus, hydroquinone increased 3-fold T. versicolor laccase activity decreasing 2- and 8-fold the yields of MnP and endoglucanase, respectively.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2010
Language Anglais
Journal information "Journal of Industrial Microbiology and Biotechnology" - Vol. 37, no. 10, p. 1091-1096 (2010)
Peer reviewed yes
Publisher Springer Heidelberg (Heidelberg)
issn 1367-5435
e-issn 1476-5535
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords White-rot basidiomycetes ; Aromatic compounds ; Laccase ; Manganese peroxidase ; Production
Links
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Bibliographic reference Elisashvili, Vladimir ; Kachlishvili, Eva ; Khardziani, Tamar ; Agathos, Spiros N.. Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes. In: Journal of Industrial Microbiology and Biotechnology, Vol. 37, no. 10, p. 1091-1096 (2010)
Permanent URL http://hdl.handle.net/2078.1/34367