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Multicentre evaluation of the Tosoh HbA1c G8 analyser

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Chapelle, Jean-Paul Maisin, Diane [UCL] Teixeira, Jelda Assink, Hans Barla, Gerhard van den Eshof, Wouter

Background: We report a Dutch-Belgian multicentre evaluation of the Tosoh HLC-723G8 glycohaemoglobin analyser, an ion-exchange HPLC instrument for the separation and quantification of haemoglobin A1c (HbA1c) in whole blood. Methods: We evaluated the analytical performances of the Tosoh G8 analyser and compared the results for blood samples with its predecessor, the Tosoh G7, and with two other widely used analysers, the Bio-Rad Variant II and Adams Arkray HA-8160. Results: Within-and between-batch imprecision [coefficient of variation (CVs)] was <0.5% and 2%, respectively, and compared favourably with the G7. The excellent performances in terms of speed (1.6 min/analysis) did not result in increased variability of the results or carry-over between samples. The method shows no interference from carbamylated haemoglobin, and recognises the presence of haemoglobinopathies, which triggers the correction of the HbA1c result. Comparison with established methods showed good correlation, not only with the G7 but also with the Variant II and HA-8160 systems. Conclusions: With respect to reproducibility, chromatographic resolution, speed of analysis and identification of Hb variants, the Tosoh G8 analyser can be considered to be state of the art. Clin Chem Lab Med 2010;48:365-71.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2010
Language Anglais
Journal information "Clinical Chemistry and Laboratory Medicine : Associated with FESCC and IFCC" - Vol. 48, no. 3, p. 365-371 (2010)
Peer reviewed yes
Publisher Walter De Gruyter & Co (Berlin)
issn 1434-6621
e-issn 1437-4331
Publication status Publié
Affiliations UCL - Cliniques universitaires Saint-Luc
UCL
Keywords diabetes ; evaluation ; glycated haemoglobin ; HbA1c ; Hb variants ; multicentre study
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Bibliographic reference Chapelle, Jean-Paul ; Maisin, Diane ; Teixeira, Jelda ; Assink, Hans ; Barla, Gerhard ; et. al. Multicentre evaluation of the Tosoh HbA1c G8 analyser. In: Clinical Chemistry and Laboratory Medicine : Associated with FESCC and IFCC, Vol. 48, no. 3, p. 365-371 (2010)
Permanent URL http://hdl.handle.net/2078.1/34153