Markers and signaling factors for stem cell differentiation to hepatocytes: lessons from developmental studies

Liver transplantation is the preferred option to treat a number of hepatic diseases in adults and children, but the number of patients on the waiting list is exceeding the number of available livers for transplantation. Hepatocytes differentiated in vitro from stem cells are a promising and renewable source of tissue for transplantation. The principles guiding programmed differentiation of stem cells to hepatocytes are largely based on knowledge gained from studies on embryonic development of the liver. How key findings in developmental biology are translated into cell culture protocols driving stepwise differentiation of hepatocytes is illustrated in this chapter.