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Electrical vagus nerve stimulation and nicotine effects in peritonitis-induced acute lung injury in rats

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Boland, Claire [UCL] Collet, Valérie [UCL] Laterre, Emmanuelle Lecuivre, Corinne [UCL] Wittebole, Xavier [UCL] Laterre, Pierre-François [UCL]

The cholinergic anti-inflammatory pathway has been identified as playing a key role in the communication between the central nervous system and the immune system during inflammation. The potential beneficial role of vagus nerve stimulation (VNS) remains to be clarified in established sepsis. We hypothesized that VNS or nicotine administration would reduce lung injury and mortality in established sepsis. We conducted a prospective, randomized experimental study. Four hours after peritonitis induction by cecal ligation and puncture (CLP), rats were randomized into three groups of seven animals according to the intervention: control group, VNS group (15 V, 2 ms, 5 Hz during 20 min), and nicotine group (400 microg/kg intraperitoneal). Survival was determined as lung injury score 4 and 8 h after CLP. Tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, IL-10, cytokine-induced neutrophil chemoattractant (CINC)-3 and thrombin-antithrombin complexes (TATc) were measured at baseline and at 4 and 8 h after CLP. Survival at 8 h was 71.4%, 100%, and 23.8% in the control, VNS, and nicotine groups, respectively (p < 0.05). All animals had lung damage but without significant difference between groups even if nicotine-treated animals tended to have a higher score than the controls (p = 0.09). Neutrophil polymorphonuclear (PMN) infiltration was more pronounced in the nicotine group compared with the VNS group (p = 0.015) but not with the controls. TNF-alpha, IL-6, IL-10, CINC-3, and TATc were elevated in all groups (NS). In this model of established sepsis, posttreatment by VNS was associated with increased survival, while nicotine administration increased lung PMN infiltration and mortality. Nicotine-induced bacterial clearance impairment and nicotine systemic effects may explain these observations.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2011
Language Anglais
Journal information "Inflammation" - Vol. 34, no. 1, p. 29-35 (2011)
Peer reviewed yes
Publisher Springer New York LLC ((United States) New York, NY)
issn 0360-3997
e-issn 1573-2576
Publication status Publié
Affiliations UCL - SSS/IREC - Institut de recherche expérimentale et clinique
UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation
UCL - SSS/IREC/MEDA - Pôle de médecine aiguë
UCL - (SLuc) Service de soins intensifs
MESH Subject Vagus Nerve - physiology ; Tumor Necrosis Factor-alpha - blood ; Rats ; Peritonitis - complications - immunology - pathology ; Peptide Hydrolases - blood ; Nicotine - pharmacology ; Neutrophil Infiltration - drug effects ; Ligation ; Kaplan-Meier Estimate ; Interleukin-6 - blood ; Interleukin-10 - blood ; Inflammation - immunology ; Electric Stimulation Therapy ; Chemokine CXCL2 - blood ; Cecum ; Antithrombin III ; Animals ; Acute Lung Injury - immunology - pathology
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Bibliographic reference Boland, Claire ; Collet, Valérie ; Laterre, Emmanuelle ; Lecuivre, Corinne ; Wittebole, Xavier ; et. al. Electrical vagus nerve stimulation and nicotine effects in peritonitis-induced acute lung injury in rats. In: Inflammation, Vol. 34, no. 1, p. 29-35 (2011)
Permanent URL http://hdl.handle.net/2078.1/30694