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Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase.

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Detheux, Michel [UCL] Vandekerckhove, J. Van Schaftingen, Emile [UCL]

cDNAs encoding the rat liver regulatory protein of glucokinase were cloned and sequenced. The deduced protein contains 568 amino acids for a molecular mass of 62,867 Da. Northern blot analysis showed the presence of a major RNA species of 2.35 kb in rat liver. No signal was observed with muscle, brain, heart, testis, intestine or spleen RNA. Recombinant regulatory protein expressed in Escherichia coli was insoluble and inactive, and was presumably contained in inclusion bodies. Western blot analysis showed that the recombinant protein was recognized by antibodies raised against regulatory protein purified from rat liver.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1993
Journal information "FEBS letters" - Vol. 321, no. 2-3, p. 111-5 (1993)
Peer reviewed yes
issn 0014-5793
Publication status Publié
Affiliation UCL
MESH Subject Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Northern ; Blotting, Western ; Carrier Proteins ; Cloning, Molecular ; DNA - genetics ; DNA Probes ; Escherichia coli - genetics ; Gene Library ; Glucokinase - metabolism ; Liver - metabolism ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; Organ Specificity ; Polymerase Chain Reaction - methods ; Proteins - genetics - isolation & purification - metabolism ; RNA - genetics - isolation & purification ; Rats ; Recombinant Proteins - isolation & purification - metabolism ; Restriction Mapping
Links
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Bibliographic reference Detheux, Michel ; Vandekerckhove, J. ; Van Schaftingen, Emile. Cloning and sequencing of rat liver cDNAs encoding the regulatory protein of glucokinase.. In: FEBS letters, Vol. 321, no. 2-3, p. 111-5 (1993)
Permanent URL http://hdl.handle.net/2078.1/29381