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Effects of antimycin, glucose deprivation, and serum on cultures of neurons, astrocytes, and neuroblastoma cells.

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Pauwels, P J Opperdoes, Frederik [UCL] Trouet, André [UCL]

The resistance of cultured mouse neuroblastoma cells, primary cultures of rat cerebellar neurons, and rat brain astrocytes to a block of aerobic metabolism was studied. Parameters such as lactate production and ATP content were measured in the presence of antimycin A and under various conditions of glucose, oxygen, and serum supply. The following conclusions can be drawn: (1) All cell types studied were characterized by an active production of lactate; (2) Incubation of the various cell types in the absence of glucose at normal oxygen tension did not affect ATP levels; (3) Respiration blocked by antimycin led to a Pasteur effect; (4) Neuroblastoma cells, but not the other cell types, were fully resistant to inhibition of respiration provided that sufficient glucose was supplied; (5) In the absence of glucose no stores of energy or utilizable substrate were present in the cell types studied when respiration was blocked; (6) In the presence of fetal calf serum anoxic neurons showed irreversible signs of degeneration.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1985
Journal information "Journal of neurochemistry" - Vol. 44, no. 1, p. 143-8 (1985)
Peer reviewed yes
issn 0022-3042
Publication status Publié
Affiliation UCL
MESH Subject Adenosine Triphosphate - metabolism ; Animals ; Antimycin A - analogs & derivatives - pharmacology ; Astrocytes - cytology ; Blood ; Cell Line ; Cells, Cultured ; Glucose ; Glycolysis ; Lactates - biosynthesis ; Lactic Acid ; Mice ; Neuroblastoma - pathology ; Neurons - cytology ; Oxygen - metabolism
Links
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Bibliographic reference Pauwels, P J ; Opperdoes, Frederik ; Trouet, André. Effects of antimycin, glucose deprivation, and serum on cultures of neurons, astrocytes, and neuroblastoma cells.. In: Journal of neurochemistry, Vol. 44, no. 1, p. 143-8 (1985)
Permanent URL http://hdl.handle.net/2078.1/27048