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Oral fluid as a medium for the detection of hepatitis B surface antigen.

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Hutse, Veronik Verhaegen, Els De Cock, Liesbet Quoilin, Sophie Vandenberghe, Hans Horsmans, Yves [UCL] Van Oyen, Herman

Currently viral antigens and antibodies are detected by traditional serological tests. However, the introduction of oral fluid as an alternative medium would allow other alternatives. The collection of oral fluid is, in comparison with venepuncture, less invasive, less painful, less expensive (i.e., no trained personal required), and safe (prevention of needle stick injuries). Also large numbers of samples can be collected easily for epidemiological purposes. Forty-three HBsAg positive and seventy-three HBsAg negative paired serum/oral fluid samples were tested. They were collected from patients attending university hospitals. The oral fluid samples were collected using the Oracol collection device and they were subjected to an IgG quantification assay to ensure their quality and quantity. The detection of HBsAg in oral fluid was carried out using a modified ETI-MAK-4 ELISA. The validation of this oral fluid test gave a sensitivity and specificity of 90.7% and 100%, respectively. The modified ETI-MAK-4 ELISA is a suitable test for oral fluid samples collected by the Oracol collection device for epidemiological purposes.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2005
Journal information "Journal of Medical Virology" - Vol. 77, no. 1, p. 53-56 (2005)
Peer reviewed yes
Publisher JohnWiley & Sons, Inc. (Hoboken)
issn 0146-6615
e-issn 1096-9071
Publication status Publié
Affiliations UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Service d'hépato-gastro-entérologie
MESH Subject Hepatitis B - immunology - virology ; Hepatitis B Antibodies - analysis ; Hepatitis B Surface Antigens - analysis - immunology ; Hepatitis B virus - chemistry - immunology ; Humans ; Saliva - immunology - virology ; Specimen Handling
Keywords Oral fluid ; Hepatitis B surface antigen ; Collection device ; ELISA
Links
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Bibliographic reference Hutse, Veronik ; Verhaegen, Els ; De Cock, Liesbet ; Quoilin, Sophie ; Vandenberghe, Hans ; et. al. Oral fluid as a medium for the detection of hepatitis B surface antigen.. In: Journal of Medical Virology, Vol. 77, no. 1, p. 53-56 (2005)
Permanent URL http://hdl.handle.net/2078.1/25649