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Particle counting immunoassay of choriogonadotropin using monoclonal antibodies.

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Halflants, M. Tasiaux, N. Van Krieken, L De Hertogh, René [UCL] Collet-Cassart, D

A latex particle immunoassay has been developed for the quantification of choriogonadotropin in human serum using two monoclonal antibodies specific for the beta-chain of the hormone. The assay, based on optical counting of monomeric particles, was achieved in 40 min and the calibration curve was linear between 10 and 200 IU/l. Intra- and interassay precisions at three different levels of the curve varied between 3.3 and 10.9%. The method was validated by comparison with two different radioimmunoassays and correlation coefficients of 0.97-0.99 were obtained.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article
Publication date 1990
Journal information "Journal of immunological methods" - Vol. 134, no. 2, p. 171-5 (1990)
Peer reviewed yes
issn 0022-1759
Publication status Publié
Affiliation UCL
MESH Subject Antibodies, Monoclonal ; Antibody Specificity ; Calibration ; Chorionic Gonadotropin - blood ; Humans ; Immunoassay - methods ; Latex Fixation Tests - methods ; Pepsin A ; Reproducibility of Results
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Bibliographic reference Halflants, M. ; Tasiaux, N. ; Van Krieken, L ; De Hertogh, René ; Collet-Cassart, D. Particle counting immunoassay of choriogonadotropin using monoclonal antibodies.. In: Journal of immunological methods, Vol. 134, no. 2, p. 171-5 (1990)
Permanent URL http://hdl.handle.net/2078.1/24745