Duverger-van Bogaert, M
Dierickx, P J
Stecca, C.
Crutzen, Marc
[UCL]
The supernatant from human Hep G2 hepatoma cells was examined for typical enzymatic activities involved in the metabolism of xenobiotics. Neither cytochrome P-450 nor b5 was detectable, but associated enzymatic activities were found especially after induction with hydrocortisone (HC) and benzanthracene (BA) suggesting that this Hep G2 supernatant contains cyt P-450 IA1 and IA2. Other critical enzymes are also present, but, as expected, at lower activities than in Aroclor 1254 rat liver S9, except for NADH and NADPH cytochrome c reductase. Results of the Ames test indicate that the induced Hep G2 supernatant is a suitable activator for the evaluation of genotoxicity of indirect mutagens.
Bibliographic reference |
Duverger-van Bogaert, M ; Dierickx, P J ; Stecca, C. ; Crutzen, Marc. Metabolic activation by a supernatant from human hepatoma cells: a possible alternative in mutagenic tests.. In: Mutation research, Vol. 292, no. 2, p. 199-204 (1993) |
Permanent URL |
http://hdl.handle.net/2078.1/24608 |