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Determinants of benzo[a]pyrene diol epoxide adducts to albumin in workers exposed to polycyclic aromatic hydrocarbons

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Tas, S. Buchet, Jean-Pierre [UCL] Lauwerys, Robert [UCL]

The present study was undertaken among 260 subjects [133 controls and 127 persons exposed to polycyclic aromatic hydrocarbons (PAHs) from two steel foundries and a graphite electrode producing plant in order to investigate the relationship between the benzo[a]-pyrene-diol epoxide adducts to albumin (BPDE-alb) and the intensity of exposure to PAHs. Blood samples were collected from each subject and BPDE-alb adduct determination was performed using a high-pressure liquid chromatography technique with fluorescence detection. Exposure to PAHs was assessed by measuring airborne concentration of 13 PAHs including benzo[a]pyrene (BaP) using personal air sampling and 1-hydroxypyrene excretion (1-HOP) in postshift urine. Significantly higher BPDE-alb adduct levels were observed in exposed workers compared to controls but wide interindividual variation was observed between subjects with the same level of exposure. BPDE-alb adduct level was weakly but significantly associated with the airborne concentrations of total PAHs (r = 0.35, P = 0.0001) and BaP (r = 0.30, P = 0.0001), and urinary 1-HOP excretion (r = 0.29, P = 0.0001). Alcohol and dietary habits, place of residence, and renal and hepatic status were not found to influence the concentration of BPDE-alb adducts significantly. However, for the same level of exposure to BaP, smokers had a higher probability of having an elevated BPDE-alb adduct level than non-smokers.(ABSTRACT TRUNCATED AT 250 WORDS)

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Journal information "International Archives of Occupational and Environmental Health" - Vol. 66, no. 5, p. 343-348 (1994)
Peer reviewed yes
Publisher Springer (Heidelberg)
issn 0340-0131
e-issn 1432-1246
Publication status Publié
Affiliations UCL - MD/ESP - Ecole de santé publique
UCL - (SLuc) Service d'anatomie pathologique
UCL - (SLuc) Centre de toxicologie clinique
MESH Subject 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - metabolism ; Adult ; Albumins - metabolism ; Humans ; Metallurgy ; Occupational Exposure ; Polycyclic Compounds - metabolism ; Pyrenes - metabolism
Keywords Polycyclic aromatic hydrocarbons ; Benzo[a]pyrene protein adducts ; Hydroxypyrene
Links
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Bibliographic reference Tas, S. ; Buchet, Jean-Pierre ; Lauwerys, Robert. Determinants of benzo[a]pyrene diol epoxide adducts to albumin in workers exposed to polycyclic aromatic hydrocarbons. In: International Archives of Occupational and Environmental Health, Vol. 66, no. 5, p. 343-348 (1994)
Permanent URL http://hdl.handle.net/2078.1/24593