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The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo.

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Dupres, Vincent [UCL] Alsteens, David [UCL] Wilk, Sabrina Hansen, Benjamin Heinisch, Jürgen J Dufrêne, Yves [UCL]

Here we report on in vivo measurement of the mechanical behavior of a cell surface sensor using single-molecule atomic force microscopy. We focus on the yeast wall stress component sensor Wsc1, a plasma membrane protein that is thought to function as a rigid probe of the cell wall status. We first map the distribution of individual histidine-tagged sensors on living yeast cells by scanning the cell surface with atomic force microscopy tips carrying nitrilotriacetate groups. We then show that Wsc1 behaves like a linear nanospring that is capable of resisting high mechanical force and of responding to cell surface stress. Both a genomic pmt4 deletion and the insertion of a stretch of glycines in Wsc1 result in substantial alterations in protein spring properties, supporting the important role of glycosylation at the extracellular serine/threonine-rich region.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2009
Journal information "Nature Chemical Biology" - Vol. 5, no. 11, p. 857-862 (2009)
Peer reviewed yes
Publisher Nature Publishing Group ((United Kingdom) London)
issn 1552-4450
e-issn 1552-4469
Publication status Publié
Affiliations UCL - FSA/MAPR - Département des sciences des matériaux et des procédés
UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Stress, Mechanical ; Solutions ; Saccharomyces cerevisiae Proteins - chemistry - physiology - ultrastructure ; Saccharomyces cerevisiae - physiology - ultrastructure ; Protein Conformation ; Models, Molecular ; Microscopy, Atomic Force - methods ; Membrane Proteins - physiology - ultrastructure ; Membrane Glycoproteins - chemistry - physiology ; Intracellular Signaling Peptides and Proteins - chemistry - physiology ; Cells, Immobilized - physiology - ultrastructure ; Cell Wall - physiology - ultrastructure ; Cell Membrane - physiology - ultrastructure ; Biosensing Techniques
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Bibliographic reference Dupres, Vincent ; Alsteens, David ; Wilk, Sabrina ; Hansen, Benjamin ; Heinisch, Jürgen J ; et. al. The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo.. In: Nature Chemical Biology, Vol. 5, no. 11, p. 857-862 (2009)
Permanent URL http://hdl.handle.net/2078.1/24557