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The lysosome turns fifty.

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De Duve, Christian [UCL]

In the course of an investigation aimed at characterizing hepatic glucose 6-phosphatase, the unrelated acid phosphatase of rat liver was serendipitously observed to be latent and particle-bound in freshly prepared homogenates. Experiments designed to elucidate this intriguing finding led, 50 years ago, to the discovery of lysosomes. This could not have happened if strict adherence to a previously set programme had been mandatory.

Document type Article de périodique (Journal article) – Historical Article, Journal Article
Publication date 2005
Journal information "Nature cell biology" - Vol. 7, no. 9, p. 847-9 (2005)
Peer reviewed yes
issn 1465-7392
Publication status Publié
Affiliation New-York - Rockefeller University
MESH Subject Acid Phosphatase - isolation & purification - metabolism ; Animals ; Biochemistry - history ; History, 20th Century ; Humans ; Liver - enzymology - ultrastructure ; Lysosomes - enzymology - ultrastructure ; Rats
Links
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  3. de Duve Christian, My Love Affair with Insulin, 10.1074/jbc.x400002200
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  5. Claude A., FRACTIONATION OF MAMMALIAN LIVER CELLS BY DIFFERENTIAL CENTRIFUGATION: I. PROBLEMS, METHODS, AND PREPARATION OF EXTRACT, 10.1084/jem.84.1.51
  6. Claude A., FRACTIONATION OF MAMMALIAN LIVER CELLS BY DIFFERENTIAL CENTRIFUGATION: II. EXPERIMENTAL PROCEDURES AND RESULTS, 10.1084/jem.84.1.61
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  9. Berthet J., De Duve C., Tissue fractionation studies. 1. The existence of a mitochondria-linked, enzymically inactive form of acid phosphatase in rat-liver tissue, 10.1042/bj0500174
  10. Berthet J., Berthet L., Appelmans F., De Duve C., Tissue fractionation studies. 2. The nature of the linkage between acid phosphatase and mitochondria in rat-liver tissue, 10.1042/bj0500182
  11. Appelmans Françoise, Wattiaux R., De Duve C., Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver, 10.1042/bj0590438
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  15. Baudhuin P., COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER: Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase, 10.1083/jcb.26.1.219
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  17. de Reuck, A. V. S. & Cameron, M. P. (eds) Ciba Foundation Symposium on Lysosomes (J. & A. Churchill Ltd, London, 1963).
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  22. Hers H. G., Gastroenterology, 48, 625 (1965)
  23. Hers, H. G. & Van Hoof, F. (eds) Lysosomes and Storage Diseases (Academic, New York, 1973).
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  27. de Duve C., Prog. Biophys. Biophys. Chem., 9, 325 (1959)
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Bibliographic reference De Duve, Christian. The lysosome turns fifty.. In: Nature cell biology, Vol. 7, no. 9, p. 847-9 (2005)
Permanent URL http://hdl.handle.net/2078.1/23935